SKU
MD05661

Avian Infectious Bronchitis virus RT-qPCR Kit

Storage Conditions:
-85°C to -15°C
Avian Infectious Bronchitis virus RT-qPCR Kit is designed for the in vitro detection of IBV genomes.
Availability
Ready to Ship
Unit Size
100 reactions
Avian Infectious Bronchitis virus (IBV) is a coronavirus that causes avian infectious bronchitis, a highly infectious disease that affects the respiratory tract, gut, kidney and reproductive system. IBV infection in chickens can lead to a decrease in egg production or quality. The virus is spread between individuals leading to a morbidity rate of 100% in bird populations that have not been vaccinated.

Avian Infectious Bronchitis virus RT-qPCR Kit is designed for the in vitro detection of IBV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to IBV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of IBV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Endogenous Control Real-time PCR Kits). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
More Information
Shipping Conditions Room Temperature
Storage Conditions -85°C to -15°C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Avian Infectious Bronchitis virus
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Duplex
Internal Control No
Features Free automatic data analyzer (Fastfinder)
Detection Method Probe Based
- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
More Information
Shipping Conditions Room Temperature
Storage Conditions -85°C to -15°C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Avian Infectious Bronchitis virus
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Duplex
Internal Control No
Features Free automatic data analyzer (Fastfinder)
Detection Method Probe Based
More Information
Shipping Conditions Room Temperature
Storage Conditions -85°C to -15°C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Avian Infectious Bronchitis virus
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Duplex
Internal Control No
Features Free automatic data analyzer (Fastfinder)
Detection Method Probe Based

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA