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SKU
MD05741
Avian orthoreovirus RT-qPCR Kit
Avian orthoreovirus RT-qPCR Kit is designed for the in vitro detection of Avian orthoreovirus genomes.
Availability
Ready to Ship
Avian orthoreovirus is an orthoreovirus from the Reoviridae family. This virus causes arthritis and tenosynovitis in poultry. It can also cause respiratory disease. Avian orthoreovirus infection is more common in young birds, because resistance begins to develop from as young as two weeks of age. It is also reportedly more common in broilers. Distribution of avian orthoreovirus is worldwide and it is present in most poultry flocks. It can be transmitted horizontally via the faeces or rarely, vertically.
Avian orthoreovirus RT-qPCR Kit is designed for the in vitro detection of Avian orthoreovirus genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to A. orthoreovirus. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of A. orthoreovirus genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Endogenous Control Real-time PCR Kits). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Avian orthoreovirus RT-qPCR Kit is designed for the in vitro detection of Avian orthoreovirus genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to A. orthoreovirus. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of A. orthoreovirus genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Endogenous Control Real-time PCR Kits). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85°C to -15°C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Avian orthoreovirus |
Sample Material | RNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Features | Free automatic data analyzer (Fastfinder) |
Detection Method | Probe Based |
- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85°C to -15°C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Avian orthoreovirus |
Sample Material | RNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Features | Free automatic data analyzer (Fastfinder) |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85°C to -15°C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Avian orthoreovirus |
Sample Material | RNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Features | Free automatic data analyzer (Fastfinder) |
Detection Method | Probe Based |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis