Bovine Leukemia virus RT-qPCR Kit

Bovine leukemia virus (BLV) is a retrovirus which causes enzootic bovine leukosis in cattle. In general, BLV causes only a benign mononucleosis-like disease in cattle. However, cases of leukemia (about 5% of infected animals) and lymphoproliferation (30%) have been reported. BLV can be transmitted between animals by any procedure that involves contact with contaminated blood.

Bovine Leukemia virus RT-qPCR Kit is designed for the in vitro detection of BLV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to BLV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of BLV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Endogenous Control Real-time PCR Kits). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.