Notification Bar Icon

Unlock 5% OFF: Shop online now and save on every purchase! Limited time offer.

Unlock 5% OFF: Shop online now and save on every purchase! Limited time offer.Learn more

Learn more
SKU
MD05651

Bovine Leukemia virus RT-qPCR Kit

Storage Conditions:
-85 °C to -15 °C
Designed for the in vitro detection of BLV. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Unit Size
100 reactions
€399.00
Bovine leukemia virus (BLV) is a retrovirus which causes enzootic bovine leukosis in cattle. In general, BLV causes only a benign mononucleosis-like disease in cattle. However, cases of leukemia (about 5% of infected animals) and lymphoproliferation (30%) have been reported. BLV can be transmitted between animals by any procedure that involves contact with contaminated blood.

Bovine Leukemia virus RT-qPCR Kit is designed for the in vitro detection of BLV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to BLV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of BLV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Bovine leukemia virus
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based
- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Bovine leukemia virus
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Bovine leukemia virus
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

Related Products