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SKU
MD05561
Crimean Congo Haemorragic Fever virus RT-qPCR Kit
Designed for the in vitro detection of CCHFV. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
The Crimean Congo hemorrhagic fever virus (CCHFV) is a member of the genus Orthonairovirus . It causes severe viral haemorrhagic fever outbreaks that have a case fatality rate of up to 40%. The virus is primarily transmitted to people from ticks and livestock animals. Human-to-human transmission can occur resulting from close contact with the blood, secretions, organs or other bodily fluids of infected persons.
Crimean Congo Haemorragic Fever virus RT-qPCR Kit is designed for the in vitro detection of CCHFV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to CCHFV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with 80% of CCHFV genomes based on a comprehensive bioinformatic analysis with all of the reference data within the NCBI database between 2018 and 2023. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Crimean Congo Haemorragic Fever virus RT-qPCR Kit is designed for the in vitro detection of CCHFV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to CCHFV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with 80% of CCHFV genomes based on a comprehensive bioinformatic analysis with all of the reference data within the NCBI database between 2018 and 2023. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Crimean Congo hemorrhagic fever virus |
Sample Material | RNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Crimean Congo hemorrhagic fever virus |
Sample Material | RNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Crimean Congo hemorrhagic fever virus |
Sample Material | RNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis