EGFP mRNA

eGFP mRNA is a ready-to-use messenger RNA (mRNA) product encoding enhanced green fluorescent protein (eGFP). It is originally derived from the jellyfish Aequorea victoria, emits green fluorescence with a peak emission at approximately 507 nm when excited at 488 nm, enabling real-time visualization by fluorescence microscopy. It is suitable for in vitro experiments and transfection in mammalian cells. The transcript is designed for expression in eukaryotic cells. It contains the nucleic acid sequence encoding the 239 amino acid EGFP protein sequence, together with non-coding regulatory elements typically required for efficient translation and stability (5′ untranslated region, 3′ untranslated region, and a 3′ poly(A) tail). The mRNA contains a 5′ Cap 1 structure, and all uridine residues are fully substituted with N1-methyl-pseudouridine (m¹Ψ) to support robust protein expression and to reduce innate immune sensing relative to unmodified mRNA. The mRNA is produced enzymatically by in vitro transcription from a DNA template, followed by capping, polyadenylation, and purification. Promoter sequences used to drive in vitro transcription from the DNA template are not part of the final mRNA product. For research use only (RUO). Not for diagnostic or therapeutic use, and not for human or animal administration.