Enterococcus faecalis qPCR Kit

Enterococcus faecalis is a Gram-positive, commensal bacterium inhabiting the gastrointestinal tracts of humans. Like other species in the genus Enterococcus, E. faecalis is found in healthy humans and can be used as a probiotic. However, in certain situations E. faecalis acts as an opportunistic pathogen causing life-threatening infections, especially in the nosocomial environment, where the naturally high levels of antibiotic resistance found in E. faecalis contribute to its pathogenicity.

Enterococcus faecalis qPCR Kit is designed for the in vitro detection of E. faecalis genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to E. faecalis. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of E. faecalis genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify E. faecalis genomes (Teng L. et al 2001).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.