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SKU
MD05011

Epstein Barr Virus (HHV4) qPCR Kit

Storage Conditions:
-85 °C to -15 °C
Designed for the in vitro detection of EBV. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Unit Size
100 reactions
€399.00
The Epstein-Barr virus (EBV), also called Human gammaherpesvirus 4, is a double-stranded DNA virus, being one of the most common viruses in humans. Infection with EBV occurs by the oral transfer of saliva and genital secretions causing infectious mononucleosis and other Epstein-Barr virus-associated lymphoproliferative diseases.

Epstein Barr Virus (HHV4) qPCR Kit is designed for the in vitro detection of EBV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to EBV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this virus. The primers and probe sequences have very high (>95%) homology with a broad range of EBV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. However, due to the inherent instability of viral genomes, it is not possible to guarantee detection of all clinical isolates. Other closely related virus are not detected. Real-time PCR detection methods have been used in several studies to identify EBV genomes (Niesters H. G. M. et al 2000).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Epstein Barr Virus (HHV4)
Sample Material DNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Epstein Barr Virus (HHV4)
Sample Material DNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Epstein Barr Virus (HHV4)
Sample Material DNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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