Glucokinase

Escherichia coli glucokinase (EC 2.7.1.2) is an intracellular enzyme responsible for the phosphorylation of glucose to glucose-6-phosphate using ATP. In contrast to those of vertebrates, glucokinases from bacteria are functionally distinct from hexokinases (EC 2.7.1.1) with respect to their more absolute specificity for glucose. The crystal structure of E. coli glucokinase has been determined (PDB coordinates 1Q18 and 1SZ2), revealing a two domain architecture with the active site located in a deep cleft formed between the two domains. The enzyme reveals a dimeric structure and presents a similar fold to human and yeast hexokinases. The Nzytech enzyme is provided in 3.2 M ammonium sulphate. Swirl the enzyme mix immediately prior to use.

Activity: 100 U/ml

Temperature and pH optimum/stability: The optimum pH and temperature are 7.5 and 25 °C, respectively.