Herpes Simplex Virus 1 & 2 qPCR Kit

Herpes simplex virus 1 & 2 (HSV1 & 2), also known as Human alphaherpesvirus 1 and Human alphaherpesvirus 2, are two members of the human Herpesviridae family, a set of viruses that produce viral infections in the majority of humans. Both HSV1 and HSV2 are very common and contagious and can be spread and transmitted through direct contact when an infected person begins shedding the virus. Since it can be transmitted through any intimate contact, it is one of the most common sexually transmitted infections.

Herpes Simplex Virus 1 & 2 qPCR Kit is designed for the in vitro detection of HSV1 & 2 genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to HSV1 & 2. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of HSV1 & 2 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. However, due to the inherent instability of viral genomes, it is not possible to guarantee detection of all clinical isolates. Other closely related virus are not detected. Real-time PCR detection methods have been used in several studies to identify HSV1 & 2 genomes (Wada K. et al 2009).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.