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SKU
HS005

High-Throughput Gene Synthesis

Storage Conditions:
-30 °C to -15 °C
For orders above 48 synthetic genes, NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes.
Availability
Produced On Demand
Unit Size
300-5000 bp
€0.00
NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes. With that in mind, we have improved standard molecular biology processes and developed innovative bioinformatics tools for gene design and codon optimization. HTP Gene Synthesis will allow you to tackle difficult research projects such as those involving determining structure-function relationships in different proteins, creating novel antibody libraries, screening gene variant libraries or optimizing protein function and expression. Our codon optimization technology – ATGenium – will allow obtaining the highest possible levels of gene expression in different expressions hosts while retaining high levels of protein solubility.
Synthetic genes can be cloned into our standard cloning vectors (pNZY30), into NZYtech's pHTP Escherichia coli expression vectors or into a selection of other bacterial, mammalian and viral expression vectors. Check the complete list of vectors below.

Features:
– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– 100% sequence accuracy guaranteed
– Cloning vector (pNZY30) with Ampicillin or Kanamycin resistance
– Possibility of cloning your synthetic gene directly into a selection of bacterial, mammalian and viral expression vectors, namely into NZYtech's pHTP E. coli expression vectors (for an additional price)
– Complete confidentiality guaranteed

Type of vector Host Vector name Bacterial resistance Resistance marker Vector size (bp) Fusion protein
Cloning Escherichia coli pNZY30-Amp Ampicilin - 2221 -
pNZY30-Kan Kanamycin - 2165 -
Expression Escherichia coli pHTP1 Kanamycin - 5332 Hexa-Histidine
pHTP2 Kanamycin - 5995 Leader less Disulfide-bond isomerase DsbC
pHTP3 Kanamycin - 5995 Mutant version of disulfide-bond isomerase Dsbc
pHTP4 Kanamycin - 6052 Disulfide-bond isomerase DsbC*
pHTP7 Kanamycin - 5968 Disulfide oxidoreductase DsbA*
pHTP8 Kanamycin - 5671 Thioredoxin (TRX)
pHTP9 Kanamycin - 6064 Green fluorescent protein (GFP)
pHTP10 Kanamycin - 6826 N-utilization substance A (NusA)
pHTP11 Kanamycin - 6004 Glutathione S-transferase (GST)
pHTP13 Kanamycin - 5509 GB1 Domain from protein G (Gb1)
pHTP14 Kanamycin - 5713 Ketosteroid isomerase (KSI)
pHTP16 Kanamycin - 6145 R. flavefaciens cellulosomal protein (Rf4)
pHTP17 Kanamycin - 6091 R. flavefaciens cellulosomal protein (Rf71)
Expression Mammalian pNZY32 (CMV) Ampicilin - 4304 -
pNZY33 (CMV BG WPRE Neo) Ampicilin Neomycin 6842 -
pNZY34 (CMV Puro) Ampicilin Puromycin 6211 -
pNZY35 (EF1 α) Ampicilin - 4931 -
Expression Viral pNZY36 (EF1 α Puro) Ampicilin Puromycin 6838 -


Delivery Specifications:
– 40-4000 ng of purified plasmid
– Gene Synthesis Report
More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Cloned gene delivery, Complex sequences, QC-100% sequence verified
Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech's proprietary codon optimization software – ATGenium - can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech's ATGenium algorithm.

ATGenium Expression levels
NZYCodon Optimiztion Algorithm
A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm
More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Cloned gene delivery, Complex sequences, QC-100% sequence verified
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More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Cloned gene delivery, Complex sequences, QC-100% sequence verified
More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Cloned gene delivery, Complex sequences, QC-100% sequence verified

MSDS

Material Safety Data Sheets
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CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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