Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization
GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy.
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies.
Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila St CBM64C
A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules.
The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans
The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major component of the plant cell wall, consists of a backbone of β-1,4-xylose (Xylp) units that are often decorated with arabinofuranose (Araf) side chains.
Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition
The breakdown of plant cell wall (PCW) glycans is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization.
The CBMomes of cellulolytic bacteria colonizing different ecological niches present distinct carbohydrate specificities
The energetic constraints posed by anaerobic ecosystems lead to the evolution of remarkable highly efficient supramolecular multi-enzyme complexes of Carbohydrate Active enZymes (CAZymes), termed Cellulosomes (