The CBMomes of cellulolytic bacteria colonizing different ecological niches present distinct carbohydrate specificities
The energetic constraints posed by anaerobic ecosystems lead to the evolution of remarkable highly efficient supramolecular multi-enzyme complexes of Carbohydrate Active enZymes (CAZymes), termed Cellulosomes (
Motivated by the advancement of genomics and the urgent need of discovery of new molecules with therapeutic interest, as are venom peptides, high throughput pipelines ( have been developed to replace traditional approaches and protocols for synthetic gene synthesis and protein production Several high throughput platforms have been used in the past decade to identify conditions for soluble protein expression or for synthetic DNA production in large scale
The breakdown of plant cell wall (PCW) glycans is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization.
The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major component of the plant cell wall, consists of a backbone of β-1,4-xylose (Xylp) units that are often decorated with arabinofuranose (Araf) side chains.
Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila St CBM64C
A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules.
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies.
GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy.
NZYTaqII DNA polymerase was kept at various temperatures from 1 to 4 weeks. After this time, samples were tested for activity in an end-point PCR assay to amplify 1-kb fragments from serial dilutions of human genomic DNA. A control was introduced, corresponding to the same batch stored at standard conditions.
Benchmarked against a total of 4 market-leading master mixes considered to be the gold-standard in qPCR Master Mixes, the NZYSupreme Multiplex One-Step RT-qPCR Probe Master Mix proved to be a leading-edge product with first-class results.