Shipping Thermal Stability Analysis: NZYTaqII DNA polymerase

Introduction

NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The enzyme was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. NZYTaq II DNA polymerase lacks 3´→ 5´ exonuclease activity and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. The enzyme was optimized to provide higher sensitivity, allowing amplification of dierent DNA fragments from as little as 5 pg of human genomic DNA. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Results

Stable performance at range of temperatures - NZYTaqII DNA polymerase was kept at various temperatures from 1 to 4 weeks. After this time, samples were tested for activity in an end-point PCR assay to amplify 1-kb fragments from serial dilutions of human genomic DNA. A control was introduced, corresponding to the same batch stored at standard conditions.

Enzyme range is not affected by exposure to room temperatures - NZYTaqII DNA polymerase was kept at various temperatures from 1 to 4 weeks. After this time, samples were tested for activity in an end-point PCR assay to amplify 5-kb fragment from serial dilutions of human genomic DNA. A control was introduced, corresponding to the same batch stored at standard conditions.

Related Products

NZYTaq II DNA polymerase

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Shipping Thermal Stability Analysis: NZYTaqII DNA polymerase