GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy.
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies.
Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila St CBM64C
A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules.