Life Science Research
The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major component of the plant cell wall, consists of a backbone of β-1,4-xylose (Xylp) units that are often decorated with arabinofuranose (Araf) side chains.
Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila St CBM64C
A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules.
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies.
GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy.
NZYTaqII DNA polymerase was kept at various temperatures from 1 to 4 weeks. After this time, samples were tested for activity in an end-point PCR assay to amplify 1-kb fragments from serial dilutions of human genomic DNA. A control was introduced, corresponding to the same batch stored at standard conditions.
Benchmarked against a total of 4 market-leading master mixes considered to be the gold-standard in qPCR Master Mixes, the NZYSupreme Multiplex One-Step RT-qPCR Probe Master Mix proved to be a leading-edge product with first-class results.
NZY Glutathione Peroxidase Kit– New Kit for diagnosis of the white muscle disease in domestic animals
White muscle disease, also known as nutritional myopathy or muscular dystrophy, is a degenerative disease of skeletal and cardiac muscles that affects all species, with particular emphasis on ruminants.
At NZYTech we intend to develop, manufacture and market solutions that simplify, accelerate and improve life sciences research. Thus, conscious of the pressures that befall on structural genomics and proteomics as a result of the dramatic increasing number of genome sequences available nowadays, we have recently developed a high-throughput (HTP) platform to efficiently clone and express a large amount of recombinant proteins that can be rapidly used in subsequent protocols for function and structure determination.
At NZYTech we are constantly developing innovative products and services in our main areas of expertise, aiming to achieve the highest standards for laboratory research and industrial analysis. We intend to serve diligently the scientific community and to provide satisfying solutions to customers worldwide. With this in mind we have recently developed a highthroughput (HTP) platform to efficiently generate hundred to thousands of expressing clones, thus leading to the rapid characterization and screening of recombinant proteins that can be applied in subsequent protocols from structure determination to industrial procedures. To achieve this, we have optimized, in a high-throughput away, most of the classical and standard methods used in molecular biology.