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A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules.

Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies.

GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy.

NZYTaqII DNA polymerase was kept at various temperatures from 1 to 4 weeks. After this time, samples were tested for activity in an end-point PCR assay to amplify 1-kb fragments from serial dilutions of human genomic DNA. A control was introduced, corresponding to the same batch stored at standard conditions.

Benchmarked against a total of 4 market-leading master mixes considered to be the gold-standard in qPCR Master Mixes, the NZYSupreme Multiplex One-Step RT-qPCR Probe Master Mix proved to be a leading-edge product with first-class results.

The high specificity of enzymes enables the analysis in complex sample matrixes without complicated sample preparation techniques.

White muscle disease, also known as nutritional myopathy or muscular dystrophy, is a degenerative disease of skeletal and cardiac muscles that affects all species, with particular emphasis on ruminants.

At NZYTech we intend to develop, manufacture and market solutions that simplify, accelerate and improve life sciences research. Thus, conscious of the pressures that befall on structural genomics and proteomics as a result of the dramatic increasing number of genome sequences available nowadays, we have recently developed a high-throughput (HTP) platform to efficiently clone and express a large amount of recombinant proteins that can be rapidly used in subsequent protocols for function and structure determination.

At NZYTech we are constantly developing innovative products and services in our main areas of expertise, aiming to achieve the highest standards for laboratory research and industrial analysis. We intend to serve diligently the scientific community and to provide satisfying solutions to customers worldwide. With this in mind we have recently developed a highthroughput (HTP) platform to efficiently generate hundred to thousands of expressing clones, thus leading to the rapid characterization and screening of recombinant proteins that can be applied in subsequent protocols from structure determination to industrial procedures. To achieve this, we have optimized, in a high-throughput away, most of the classical and standard methods used in molecular biology.

Efficacy of de novo gene synthesis largely depends on the quality of overlapping oligonucleotides used as template for PCR assembly. The error rate associated with current gene synthesis protocols limits the efficient and accurate production of synthetic genes, both in the small and large scales. Here, we analysed the ability of different endonuclease enzymes, which specifically recognize and cleave DNA mismatches resulting from incorrect impairments between DNA strands, to remove mutations accumulated in synthetic genes.