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SKU
AK00171

L-Arginine/Urea/Ammonia, UV method

Storage Conditions:
2 °C to 8 °C
This rapid and simple stereo-specific enzymatic method is used for the simultaneous determination of L-arginine, urea and ammonia (yeast available nitrogen, YAN) in foodstuffs and in the wine industry (grape juice, fermenting must and fermenting wine). This kit is suitable for both manual and micro-volume formats.
Availability
On Demand
Unit Size
50 tests of each
€239.00
Enzymatic method for the determination of L-arginine, urea and ammonia (Yeast Available Nitrogen -YAN). Based on the spectrophotometric measurement of NADPH consumed through the reactions, after addition of arginase (ARG), urease (URE) and glutamate dehydrogenase (GlDH).

This rapid and simple stereo-specific enzymatic method is used for the simultaneous determination of L-arginine, urea and ammonia (yeast available nitrogen, YAN) in foodstuffs and in the wine industry (grape juice, fermenting must and fermenting wine). This kit is suitable for both manual and micro-volume formats.

The determination of YAN (L-arginine, urea and ammonia) is an indicator of the amount of nitrogen available for yeast growth. YAN comprises three components: free ammonium ions, amino nitrogen from free amino acids and from the side chain of Larginine. As the concentrations of these compounds vary widely, each compound must be determined for the accurate calculation of YAN, which is possible by using this Nzytech’s kit. In wine industry, the YAN can be managed by addition of nutrient supplements to grape juice prior to/during fermentation. Nzytech has also analytical kits for the determination of ammonia (AK000091) and both urea and ammonia (AK000101).
More Information
Shipping Conditions Room Temperature
Storage Conditions 2 °C to 8 °C
Application Fermentation, Wine
Features Detection limit: 0.07 mg/L ammonia, Detection limit: 0.13 mg/L urea, Detection limit: 0.37 mg/L L-arginine, Range: 10-70 mg/L ammonia, Range: 20-140 mg/L urea, Range: 50-400 mg/L L-arginine
Detection Method UV Method
Analyte Ammonia, L-Arginine, Urea
– Solution 1 (assay buffer)

– Tablets 2 (cofactor NADPH)

– Suspension 3 (enzyme GlDH)

– Suspension 4. (enzume URE)

– Suspension 5 (enzyme ARG)

– Solution 6 (Ammonia standard solution- 0.04 mg/mL)

– Powder 7 (L-Arginine standard)
More Information
Shipping Conditions Room Temperature
Storage Conditions 2 °C to 8 °C
Application Fermentation, Wine
Features Detection limit: 0.07 mg/L ammonia, Detection limit: 0.13 mg/L urea, Detection limit: 0.37 mg/L L-arginine, Range: 10-70 mg/L ammonia, Range: 20-140 mg/L urea, Range: 50-400 mg/L L-arginine
Detection Method UV Method
Analyte Ammonia, L-Arginine, Urea
More Information
Shipping Conditions Room Temperature
Storage Conditions 2 °C to 8 °C
Application Fermentation, Wine
Features Detection limit: 0.07 mg/L ammonia, Detection limit: 0.13 mg/L urea, Detection limit: 0.37 mg/L L-arginine, Range: 10-70 mg/L ammonia, Range: 20-140 mg/L urea, Range: 50-400 mg/L L-arginine
Detection Method UV Method
Analyte Ammonia, L-Arginine, Urea

MSDS

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CoA

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