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SKU
AK00111

L-Glutamine/Ammonia, UV method

Storage Conditions:
2 °C to 8 °C
This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products
Availability
On Demand
Unit Size
50 tests of each
€209.00
Enzymatic method for the determination of L-glutamine and ammonia. Based on the spectrophotometric measurement of NADP+ formed through the combined action of glutaminase (GLT) and glutamate dehydrogenase (GlDH).

This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products.

L-Glutamine is an essential nutrient of cell culture media. However, the incorporation of this labile amino acid in growth media leads to its spontaneous break down to L-glutamate and free ammonium ions. The released ammonium ions are very toxic to cells. To overcome these issues, either pre-formulated growth media are used strictly within their recommended shelf-lives or L-glutamine is added just before use. In either case, monitoring of L-glutamine and ammonia is frequently performed both prior to, and during culturing. Related analytical kits are also available from Nzytech for ammonia (AK00091) and urea/ammonia (AK00101).
More Information
Shipping Conditions Room Temperature
Storage Conditions 2 °C to 8 °C
Application Fermentation, Food
Features Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia
Detection Method UV Method
Analyte Ammonia, L-Glutamine
– Solution 1 (assay buffer 1)

– Solution 2 (assay buffer 2)

– Tablets 3 (cofactor NADPH)

– Suspension 4 (enzyme GLT)

– Suspension 5 (enzyme GlDH)

– Solution 6 (Ammonia standard solution – 0.04 mg/mL)

– Powder 7 (L-Glutamine control powder)
More Information
Shipping Conditions Room Temperature
Storage Conditions 2 °C to 8 °C
Application Fermentation, Food
Features Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia
Detection Method UV Method
Analyte Ammonia, L-Glutamine
More Information
Shipping Conditions Room Temperature
Storage Conditions 2 °C to 8 °C
Application Fermentation, Food
Features Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia
Detection Method UV Method
Analyte Ammonia, L-Glutamine

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

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