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SKU
AK00111
L-Glutamine/Ammonia, UV method
This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products
Availability
On Demand
Enzymatic method for the determination of L-glutamine and ammonia. Based on the spectrophotometric measurement of NADP+ formed through the combined action of glutaminase (GLT) and glutamate dehydrogenase (GlDH).
This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products.
L-Glutamine is an essential nutrient of cell culture media. However, the incorporation of this labile amino acid in growth media leads to its spontaneous break down to L-glutamate and free ammonium ions. The released ammonium ions are very toxic to cells. To overcome these issues, either pre-formulated growth media are used strictly within their recommended shelf-lives or L-glutamine is added just before use. In either case, monitoring of L-glutamine and ammonia is frequently performed both prior to, and during culturing. Related analytical kits are also available from Nzytech for ammonia (AK00091) and urea/ammonia (AK00101).
This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products.
L-Glutamine is an essential nutrient of cell culture media. However, the incorporation of this labile amino acid in growth media leads to its spontaneous break down to L-glutamate and free ammonium ions. The released ammonium ions are very toxic to cells. To overcome these issues, either pre-formulated growth media are used strictly within their recommended shelf-lives or L-glutamine is added just before use. In either case, monitoring of L-glutamine and ammonia is frequently performed both prior to, and during culturing. Related analytical kits are also available from Nzytech for ammonia (AK00091) and urea/ammonia (AK00101).
Shipping Conditions | Room Temperature | Storage Conditions | 2 °C to 8 °C | Application | Fermentation, Food | Features | Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia | Detection Method | UV Method | Analyte | Ammonia, L-Glutamine |
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– Solution 1 (assay buffer 1)
– Solution 2 (assay buffer 2)
– Tablets 3 (cofactor NADPH)
– Suspension 4 (enzyme GLT)
– Suspension 5 (enzyme GlDH)
– Solution 6 (Ammonia standard solution – 0.04 mg/mL)
– Powder 7 (L-Glutamine control powder)
– Solution 2 (assay buffer 2)
– Tablets 3 (cofactor NADPH)
– Suspension 4 (enzyme GLT)
– Suspension 5 (enzyme GlDH)
– Solution 6 (Ammonia standard solution – 0.04 mg/mL)
– Powder 7 (L-Glutamine control powder)
Shipping Conditions | Room Temperature | Storage Conditions | 2 °C to 8 °C | Application | Fermentation, Food | Features | Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia | Detection Method | UV Method | Analyte | Ammonia, L-Glutamine |
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Shipping Conditions | Room Temperature | Storage Conditions | 2 °C to 8 °C | Application | Fermentation, Food | Features | Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia | Detection Method | UV Method | Analyte | Ammonia, L-Glutamine |
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CoA
Certificate of Analysis