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Acetyl xylan esterase 7A, Bacillus subtilis

Storage Conditions:
-30°C to -15°C
Acetyl xylan esterase 7A (BsAxe7A), assigned the E.C. number 3.1.1.72 and 3.1.1.41, is a derivative of Bacillus subtilis. It is an esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylooligosaccharides and cephalosporin C. The recombinant BsAxe7A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 7 (CE7) enzyme (see more details at www.cazy.org).
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Acetyl xylan esterase 7A (BsAxe7A), assigned the E.C. number 3.1.1.72 and 3.1.1.41, is a derivative of Bacillus subtilis. It is an esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylooligosaccharides and cephalosporin C. The recombinant BsAxe7A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 7 (CE7) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Carbohydrate Esterase
Activity Acetyl xylan esterases
Family CE7
EC number 3.1.1.72 and 3.1.1.41
Organism Bacillus subtilis
Architecture CE7
Optimal pH 6.5
Optimal Temperature 37°C
Substrates 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-Penta-acetyl-xylobiose, 3′-O-Acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C
Mitsushima et al. (1995) Appl Environ Microbiol. 61(6):2224-2229.
Vincent et al. (2003) J. Mol. Biol. 330, 593-606.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Carbohydrate Esterase
Activity Acetyl xylan esterases
Family CE7
EC number 3.1.1.72 and 3.1.1.41
Organism Bacillus subtilis
Architecture CE7
Optimal pH 6.5
Optimal Temperature 37°C
Substrates 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-Penta-acetyl-xylobiose, 3′-O-Acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Carbohydrate Esterase
Activity Acetyl xylan esterases
Family CE7
EC number 3.1.1.72 and 3.1.1.41
Organism Bacillus subtilis
Architecture CE7
Optimal pH 6.5
Optimal Temperature 37°C
Substrates 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-Penta-acetyl-xylobiose, 3′-O-Acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C

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