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Acetyl xylan esterase 7A, Bacillus subtilis
Acetyl xylan esterase 7A (BsAxe7A), assigned the E.C. number 3.1.1.72 and 3.1.1.41, is a derivative of Bacillus subtilis. It is an esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylooligosaccharides and cephalosporin C. The recombinant BsAxe7A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 7 (CE7) enzyme (see more details at www.cazy.org).
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Produced On Demand
Acetyl xylan esterase 7A (BsAxe7A), assigned the E.C. number 3.1.1.72 and 3.1.1.41, is a derivative of Bacillus subtilis. It is an esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylooligosaccharides and cephalosporin C. The recombinant BsAxe7A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 7 (CE7) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
| Shipping Conditions | Shipping: Dry Ice to RT | Storage Conditions | -30 °C to -15 °C | Class | Carbohydrate Esterase | Activity | Acetyl xylan esterases | Family | CE7 | EC number | 3.1.1.72 and 3.1.1.41 | Organism | Bacillus subtilis | Architecture | CE7 | Optimal pH | 6.5 | Optimal Temperature | 37 °C | Substrates | 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-Penta-acetyl-xylobiose, 3′-O-Acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C |
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Mitsushima et al. (1995) Appl Environ Microbiol. 61(6):2224-2229.
Vincent et al. (2003) J. Mol. Biol. 330, 593-606.
Vincent et al. (2003) J. Mol. Biol. 330, 593-606.
| Shipping Conditions | Shipping: Dry Ice to RT | Storage Conditions | -30 °C to -15 °C | Class | Carbohydrate Esterase | Activity | Acetyl xylan esterases | Family | CE7 | EC number | 3.1.1.72 and 3.1.1.41 | Organism | Bacillus subtilis | Architecture | CE7 | Optimal pH | 6.5 | Optimal Temperature | 37 °C | Substrates | 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-Penta-acetyl-xylobiose, 3′-O-Acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C |
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| Shipping Conditions | Shipping: Dry Ice to RT | Storage Conditions | -30 °C to -15 °C | Class | Carbohydrate Esterase | Activity | Acetyl xylan esterases | Family | CE7 | EC number | 3.1.1.72 and 3.1.1.41 | Organism | Bacillus subtilis | Architecture | CE7 | Optimal pH | 6.5 | Optimal Temperature | 37 °C | Substrates | 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-Penta-acetyl-xylobiose, 3′-O-Acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C |
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