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SKU
MB40001
NZY First-Strand cDNA Synthesis Flexible Pack
NZY First-Strand cDNA Synthesis Flexible Pack includes all the necessary components to synthesize first-strand cDNA from an RNA template. In addition, it offers the possibility to choose the primer to initiate the reaction.
NZY First-Strand cDNA Synthesis Flexible Pack is an optimized pack that includes the following components: the modified recombinant form of the Moloney Murine Leukemia Virus (MMuLV) Reverse Transcriptase (RNase H minus), which synthesizes the complementary DNA strand using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50 °C); an optimized Reverse Transcriptases buffer; the NZY Ribonuclease Inhibitor for RNA protection against degradation due to ribonuclease contamination; a dNTPs mix; random hexamers and oligo(dT)18 primers; and finally, an E. coli RNase H to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This flexible pack offers the possibility to choose the primer to initiate the reaction: you can choose either the primers provided in separate tubes, or a gene-specific primer. The pack is sufficient for up to 200 first-strand cDNA synthesis reactions and it is optimized for RT reactions over a wide range of total RNA concentrations from 10 pg to 5 μg. Downstream applications include real-time PCR, standard PCR and microarrays.
| Shipping Conditions | Blue Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Fast, Flexible primer selection, High-yield, Thermostable | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 50 °C | Protocol time | Approx. 60 min. |
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– NZY Reverse Transcriptase (200 U/μL)
– 10x Reaction buffer for Reverse Transcriptases
– NZY Ribonuclease Inhibitor (40 U/µL)
– dNTP Mix (10 mM each)
– Random hexamer mix (50 ng/µL)
– Oligo(dT)18 primer mix (50 μM)
– NZY RNase H (E. coli) (5 U/μL)
– DEPC-treated H2O
– 10x Reaction buffer for Reverse Transcriptases
– NZY Ribonuclease Inhibitor (40 U/µL)
– dNTP Mix (10 mM each)
– Random hexamer mix (50 ng/µL)
– Oligo(dT)18 primer mix (50 μM)
– NZY RNase H (E. coli) (5 U/μL)
– DEPC-treated H2O
| Shipping Conditions | Blue Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Fast, Flexible primer selection, High-yield, Thermostable | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 50 °C | Protocol time | Approx. 60 min. |
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TLR4 Deficiency Affects the Microbiome and Reduces Intestinal Dysfunctions and Inflammation in Chronic Alcohol-Fed Mice.
Cuesta C, Pascual M, Pérez-Moraga R, Rodríguez-Navarro I, García-García F, Ureña-Peralta J, Guerri C.
Frontiers in Immunology, 2021.
Ethylene Action Inhibition Improves Adventitious Root Induction in Adult Chestnut Tissues.
Castro-Camba R, Neves M, Correia S, Canhoto J, Vielba J, Sánchez C.
Plants (MDPI), 2024.
Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav.
Neves M, Correia S, Canhoto J.
Plants (MDPI), 2023.
Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar.
Cordeiro D, Canhoto J, Correia S.
BMC Plant Biology (Springer Nature), 2025.
Temporal and spatial resolution of magnetosome degradation at the subcellular level in a 3D lung carcinoma model.
Gubieda A, Gandarias L, Pósfai M, Pattammattel A, Fdez-Gubieda L, Abad-Díaz-de-Cerio A, García-Prieto A.
Journal of Nanobiotechnology (Springer Nature), 2024.
| Shipping Conditions | Blue Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Fast, Flexible primer selection, High-yield, Thermostable | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 50 °C | Protocol time | Approx. 60 min. |
|---|
| Shipping Conditions | Blue Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Fast, Flexible primer selection, High-yield, Thermostable | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 50 °C | Protocol time | Approx. 60 min. |
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SDS
Safety Data Sheets
CoA
Certificate of Analysis
