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NZY-A Speedy PCR cloning kit

Storage Conditions:
Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
NZY-A Speedy PCR cloning kit was designed to carry out fast and efficient cloning of PCR products with 3´-A overhangs, which result from amplifications using non-proofreading DNA polymerases.
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Ready to Ship
Dry Ice
From €169.00
NZY-A Speedy PCR cloning kit was designed to carry out fast and
efficient cloning of PCR products with 3´-A overhangs, which result
from amplifications using non-proofreading DNA polymerases such
as NZYTaq II DNA polymerase. This methodology combines the
efficiency of an improved ligation buffer with the speed of Speedy
Ligase to allow a rapid ligation between the vector and the PCR
product in only 5 minutes at room temperature (18-25 °C). The
cloning vector was prepared by cutting NZYtech´s pNZY28 with
EcoR V and adding a 3´ terminal thymidine at both ends. These
single 3´-T overhangs improve the efficiency of ligation of a PCR
product into plasmids by preventing re-circularization of the vector
and providing compatible overhangs for PCR products generated by
non-proofreading thermostable polymerases. Vector pNZY28
contains multiple restriction sites within the multiple cloning
region. However, vector digestion with EcoR I or BamH I allows the
release of the PCR product since the vector cloning region is flanked
by recognition sites of both enzymes.
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
Application TA Cloning
Features Blue/White screening, Fast
Product Category DNA Cloning & Mutagenesis
Protocol time Approx. 10 min
– NZY-A Speedy Buffer (4x)
– pNZY28-A vector (50 ng/µL)
– Speedy Ligase (5 U/µL)
– NZY-A positive control insert (50 ng/µL)
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)

(*) only provided in MB13701 kit
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
Application TA Cloning
Features Blue/White screening, Fast
Product Category DNA Cloning & Mutagenesis
Protocol time Approx. 10 min
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
Application TA Cloning
Features Blue/White screening, Fast
Product Category DNA Cloning & Mutagenesis
Protocol time Approx. 10 min

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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