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NZY-A Speedy PCR cloning kit
NZY-A Speedy PCR cloning kit was designed to carry out fast and efficient cloning of PCR products with 3´-A overhangs, which result from amplifications using non-proofreading DNA polymerases.
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Dry Ice
NZY-A Speedy PCR cloning kit was designed to carry out fast and
efficient cloning of PCR products with 3´-A overhangs, which result
from amplifications using non-proofreading DNA polymerases such
as NZYTaq II DNA polymerase. This methodology combines the
efficiency of an improved ligation buffer with the speed of Speedy
Ligase to allow a rapid ligation between the vector and the PCR
product in only 5 minutes at room temperature (18-25 °C). The
cloning vector was prepared by cutting NZYtech´s pNZY28 with
EcoR V and adding a 3´ terminal thymidine at both ends. These
single 3´-T overhangs improve the efficiency of ligation of a PCR
product into plasmids by preventing re-circularization of the vector
and providing compatible overhangs for PCR products generated by
non-proofreading thermostable polymerases. Vector pNZY28
contains multiple restriction sites within the multiple cloning
region. However, vector digestion with EcoR I or BamH I allows the
release of the PCR product since the vector cloning region is flanked
by recognition sites of both enzymes.
efficient cloning of PCR products with 3´-A overhangs, which result
from amplifications using non-proofreading DNA polymerases such
as NZYTaq II DNA polymerase. This methodology combines the
efficiency of an improved ligation buffer with the speed of Speedy
Ligase to allow a rapid ligation between the vector and the PCR
product in only 5 minutes at room temperature (18-25 °C). The
cloning vector was prepared by cutting NZYtech´s pNZY28 with
EcoR V and adding a 3´ terminal thymidine at both ends. These
single 3´-T overhangs improve the efficiency of ligation of a PCR
product into plasmids by preventing re-circularization of the vector
and providing compatible overhangs for PCR products generated by
non-proofreading thermostable polymerases. Vector pNZY28
contains multiple restriction sites within the multiple cloning
region. However, vector digestion with EcoR I or BamH I allows the
release of the PCR product since the vector cloning region is flanked
by recognition sites of both enzymes.
| Shipping Conditions | Dry Ice | Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
Application | TA Cloning | Features | Blue/White screening, Fast | Product Category | DNA Cloning & Mutagenesis | Protocol time | Approx. 10 min. |
|---|
– NZY-A Speedy Buffer (4x)
– pNZY28-A vector (50 ng/µL)
– Speedy Ligase (5 U/µL)
– NZY-A positive control insert (50 ng/µL)
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)
(*) only provided in MB13701 kit
– pNZY28-A vector (50 ng/µL)
– Speedy Ligase (5 U/µL)
– NZY-A positive control insert (50 ng/µL)
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)
(*) only provided in MB13701 kit
| Shipping Conditions | Dry Ice | Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
Application | TA Cloning | Features | Blue/White screening, Fast | Product Category | DNA Cloning & Mutagenesis | Protocol time | Approx. 10 min. |
|---|
| Shipping Conditions | Dry Ice | Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
Application | TA Cloning | Features | Blue/White screening, Fast | Product Category | DNA Cloning & Mutagenesis | Protocol time | Approx. 10 min. |
|---|
CoA
Certificate of Analysis
