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SKU
MB42701
T4 ssRNA Ligase
T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.
Availability
Ready to Ship
Dry Ice
T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Note that the minimal substrate for T4 ssRNA Ligase is a nucleoside 3’,5’-biphosphate in intermolecular reaction and oligonucleotide of 8 bases in intramolecular reaction. The enzyme may be used for the ligation of ssRNA and DNA, 3´-termini of RNA labelling, synthesis of single-stranded oligoribo- and oligodeoxyribonucleotides and specific modifications of tRNAs.
Applications:
– Ligation of ssRNA and DNA
– 3´-end labelling of single strand RNA
– Synthesis of single-stranded oligoribonucleotides and oligodeoxyribonucleotides
– Specific modifications of tRNAs
Applications:
– Ligation of ssRNA and DNA
– 3´-end labelling of single strand RNA
– Synthesis of single-stranded oligoribonucleotides and oligodeoxyribonucleotides
– Specific modifications of tRNAs
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | RNA ligation |
Product Category | Ligases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
– T4 ssRNA Ligase (10 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | RNA ligation |
Product Category | Ligases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | RNA ligation |
Product Category | Ligases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
CoA
Certificate of Analysis