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SKU
MB42902
Exonuclease I (E. coli)
Exonuclease I (E. coli) is a typical DNA specific exonuclease that catalyses the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction.
Exonuclease I (E. coli) is a typical DNA specific exonuclease that catalyses the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction. The enzyme may be used to remove single-stranded primers in PCR reactions prior to Sanger DNA sequencing or SNP analysis nested PCR reactions. Exonuclease I (E. coli) may be used to generally remove linear single-stranded DNA, leaving behind double-stranded DNA in the sample.
Applications:
– Removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction
– Removal of single-stranded primers in PCR mixture prior to Sanger DNA sequencing
– Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
Applications:
– Removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction
– Removal of single-stranded primers in PCR mixture prior to Sanger DNA sequencing
– Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
| Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Activity | 3′ → 5′ ssDNA nuclease | Product Category | Nucleases | Reaction Temperature | 37 °C | Heat Inactivation | 80 °C | Concentration | 20 U/μL |
|---|
– Exonuclease I (E.coli) (20 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
| Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Activity | 3′ → 5′ ssDNA nuclease | Product Category | Nucleases | Reaction Temperature | 37 °C | Heat Inactivation | 80 °C | Concentration | 20 U/μL |
|---|
| Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Activity | 3′ → 5′ ssDNA nuclease | Product Category | Nucleases | Reaction Temperature | 37 °C | Heat Inactivation | 80 °C | Concentration | 20 U/μL |
|---|
SDS
Safety Data Sheets
CoA
Certificate of Analysis
