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NZYMidiprep
NZMidiprep kit allows for rapid preparation of medium-scale highly pure plasmid DNA from E. coli strains.
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NZYMidiprep kit is designed for the rapid, medium-scale preparation of highly pure plasmid DNA (typically 100 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYtech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 100 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Midiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
– Buffer M1
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Midi Columns
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Midi Columns
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Midiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
da Cunha M, Pais SV, Bugalhão JN, Mota LJ
PLoS One, 2017
Structure-based virtual screening toward the discovery of novel inhibitors of the DNA repair activity of the human apurinic/apyrimidinic endonuclease 1
Guerreiro PS, Estácio SG, Antunes F, Fernandes AS, Pinheiro PF, Costa JG, Castro M, Miranda JP, Guedes RC, Oliveira NG.
Chem Biol Drug Des, 2016
da Cunha M, Pais SV, Bugalhão JN, Mota LJ
PLoS One, 2017
Structure-based virtual screening toward the discovery of novel inhibitors of the DNA repair activity of the human apurinic/apyrimidinic endonuclease 1
Guerreiro PS, Estácio SG, Antunes F, Fernandes AS, Pinheiro PF, Costa JG, Castro M, Miranda JP, Guedes RC, Oliveira NG.
Chem Biol Drug Des, 2016
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Midiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
Isolation of high-copy number plasmid DNA (in duplicate) using NZYMidiprep (1) or a competitor plasmid purification kit (2). Lane M: NZYDNA Ladder III.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Midiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Midiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
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Certificate of Analysis
