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NZYMaxiprep
NZYMaxiprep kit allows for rapid preparation of large-scale highly pure plasmid DNA from E. coli strains.
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NZYMaxiprep kit is designed for the rapid, large-scale preparation of highly pure plasmid DNA (typically 500 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYtech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 500 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
– Buffer M1
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Maxi Columns
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Maxi Columns
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
HPV-16 targeted DNA vaccine expression: The role of purification
Almeida AM, Tomás J, Pereira P, Queiroz JA, Sousa F, Sousa Â.
Biotechnol Prog, 2018
Chromatographic HPV-16 E6/E7 plasmid vaccine purification employing L-histidine and 1-benzyl-L-histidine affinity ligands
Amorim LFA, Gaspar R, Pereira P, Černigoj U, Sousa F, Queiroz JA, Sousa Â.
Electrophoresis, 2017
Almeida AM, Tomás J, Pereira P, Queiroz JA, Sousa F, Sousa Â.
Biotechnol Prog, 2018
Chromatographic HPV-16 E6/E7 plasmid vaccine purification employing L-histidine and 1-benzyl-L-histidine affinity ligands
Amorim LFA, Gaspar R, Pereira P, Černigoj U, Sousa F, Queiroz JA, Sousa Â.
Electrophoresis, 2017
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
Isolation and subsequent restriction analysis of two different high-copy number DNA plasmids (1 and 2). DNA was purified with NZYMaxiprep from E. coli cultures grown in LB medium. Subsequently, 1 μg of each prep was digested with Eco RI and samples were separated through electrophoresis onto a 1% agarose gel. Lane M: NZYDNA Ladder III.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 90 min |
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Certificate of Analysis
