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NZY Plant/Fungi gDNA Isolation kit
NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples by means of silica-based spin column.
NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples. The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses two optimized lysis buffers systems based on the established CTAB and SDS methods. NZY Homogenization columns are included in this kit for conveniently clearing the crude lysates. The clear flow-through is mixed with Binding buffer PN for optimal binding of DNA to the silica membrane. Then, the DNA is selectively absorbed into the NZYSpin Plant Column and others impurities such as proteins and salts are removed during the washing steps.
The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Fungi, Plant tissue | Scale | Miniprep | Features | Manual | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
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– Buffer PNL1
– Buffer PNL2
– Buffer PNL3
– Buffer PN
– Buffer PNW1
– Buffer PNW2 (concentrate)
– Buffer PNE
– RNase A(lyophilized)
– NZYHomogenization columns (purple rings)
– NZYSpin Plant columns (green rings)
– Collection tubes (2 mL)
– Buffer PNL2
– Buffer PNL3
– Buffer PN
– Buffer PNW1
– Buffer PNW2 (concentrate)
– Buffer PNE
– RNase A(lyophilized)
– NZYHomogenization columns (purple rings)
– NZYSpin Plant columns (green rings)
– Collection tubes (2 mL)
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Fungi, Plant tissue | Scale | Miniprep | Features | Manual | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
Metabolically engineered plant cell cultures as biofactories for the production of high-value carotenoids astaxanthin and canthaxanthin.
Rebelo B, Ventura M, Abranches R
Nature Scientific Reports, 2025.
An effective culturomics approach to study the gut microbiota of mammals.
Pereira A, Cunha M.
Journal of Immunological Methods (Elsevier), 2025.
Improvement of β-Xylosidase and Endoxylanase Activities in Talaromyces amestolkiae by Genetic Manipulation of the Transcriptional Activator XlnR.
Pozo-Rodríguez A, Peñalva M, Barriuso J, Espeso E, Martínez M.
Microbial Biotechnology, 2025.
It's what's inside that counts: DNA-barcoding of porcini (Boletus sp., Basidiomycota) commercial products reveals product mislabelling.
Rivas-Ferreiro M, Otero A, Morán P.
Food Control (ELsevier), 2023.
Diversity of Culture Microorganisms from Portuguese Sweet Cherries.
Gonçalves A, Falcão A, Alves G, Silva L, Flores-Fénix J.
MDPI, 2023.
Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites.
Alenquer M, Vale-Costa S, Etibor T, Ferreira F, Sousa A, Amorim M.
Nature Communications, 2019.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Fungi, Plant tissue | Scale | Miniprep | Features | Manual | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
Protocol Overview for isolating gDNA from plant:
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Fungi, Plant tissue | Scale | Miniprep | Features | Manual | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Fungi, Plant tissue | Scale | Miniprep | Features | Manual | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
SDS
Safety Data Sheets
CoA
Certificate of Analysis
