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NZYMaxiprep Endotoxin free
NZYMaxiprep Endotoxin Free allows for rapid preparation of highly pure plasmid from E. coli virtually free of endotoxins.
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NZYMaxiprep Endotoxin Free kit was designed for the rapid, medium-scale preparation of highly pure plasmid DNA (typically 500 µg) from recombinant Escherichia coli strains, virtually free of endotoxins (<0.1 EU/µg plasmid DNA). Plasmid DNA binds selectively to NZYMaxi columns-Endotoxin Free charged with a silica-based anion-exchange resin. Effective washes allow the complete removal of contaminants, such as proteins, RNA, salts, nucleotides, oligos and endotoxins. The purified nucleic acid products are suitable to use in transfection of very sensitive cells like primary or neuronal cells. Using NZYMaxiprep Endotoxin Free kit, in two efficient washing steps, the plasmid DNA is eluted completely endotoxin free.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15°C to 25°C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transfection, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Endotoxin Free, Manual |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 120 min |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15°C to 25°C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transfection, Transformation |
| Starting Material | E. coli culture |
| Scale | Maxiprep |
| Features | Endotoxin Free, Manual |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 120 min |
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