Speedy NZYTaq 2x Colourless Master Mix

Speedy NZYTaq 2× Colourless Master Mix is a premixed ready-touse solution containing Speedy NZYTaq DNA polymerase (MB403), a recombinant DNA polymerase displaying a faster polymerization reaction than any other conventional non-proofreading enzyme. Only 5 seconds are required for the successful synthesis of 1 kb size DNA. The enzyme retains its speed (5 sec/kb) when amplifying fragments up to around 2-3 kb. Successful amplification of higher DNA fragments up to 6 kb in size can be reached using a 10 sec/kb extension step. Faster PCR can be further achieved by increasing the primers melting temperature, which increases PCR annealing temperature, thus allowing combining the annealing and extension PCR steps during PCR cycling (see below). The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. For highly sensitive downstream applications it is recommended to purify the amplified PCR product using NZYGelpure (MB011) before usage in subsequent protocols. NZYSpeedy DNA polymerase lacks 3'→5' exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech's NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).