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NZYSupreme qPCR Green Master Mix (2x), ROX

Storage Conditions:
-85 °C to -15 °C
NZYSupreme qPCR Green Master Mix, ROX is a high-performing and easy-to-use mixture for real-time PCR. It features a hot-start enzyme control mechanism and it is optimized for DNA-intercalating dye detection on various instruments.
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NZYSupreme qPCR Green Master Mix (2x) is an optimized and highly efficient reaction mixture developed for real-time PCR. This master mix was engineered with a hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme qPCR Green Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. These combinations guarantee that NZYSupreme qPCR Green Master Mix (2x) delivers ultra-sensitivity coupled with highly reproducible and fast real-time PCR protocols. It was designed to amplify targets for accurate gene expression analysis. The master mix is provided as a 2x reaction mixture that contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. NZYSupreme qPCR Green Master Mix (2x) is ready-to-use and only requires primers and DNA template addition. It is optimized for intercalating green dye detection on different instruments.
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Intercalating dye
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 60 min.
Hot Start Yes
– NZYSupreme qPCR Green Master Mix (2x), ROX
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Intercalating dye
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 60 min.
Hot Start Yes

Structural basis for Gemin5 decamer-mediated mRNA binding.Guo Q, Zhao S, Francisco-Velilla R, Zhang J, Embarc-Buh A, Abellan S, Lv M, Tang P, Gong Q, Shen H, Sun L, Yao X, Min J, Shi Y, Martínez-Salas E, Zhang K & Xu C.Nature Communications, 2022. 


In vitro selection of DNA aptamers against staphylococcal enterotoxin A.Oliveira R, Pinho E, Barros MM, Azevedo NF & Almeida C.Nature Scientific Reports, 2024. 


Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens.Calvo-Villamañán A, Sastre-Dominguez J, Barrera-Martín Á, Costas C & San Millán Á.Nature Communications, 2025. 

More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Intercalating dye
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 60 min.
Hot Start Yes
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Intercalating dye
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 60 min.
Hot Start Yes

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