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NZYSupreme Multiplex qPCR Probe Master Mix (2x)
NZYSupreme Multiplex qPCR Probe Master Mix (2x) is an optimized and highly efficient reaction mixture developed for real-time PCR.
NZYSupreme Multiplex qPCR Probe Master Mix (2x) is an optimized and highly efficient reaction mixture developed for real-time PCR. The master mix, formulated at a 2× concentration, contains all components necessary for multiplex qPCR amplification (including enzyme, dNTPs, stabilizers and enhancers). Depending on the real-time thermal cycler used, assays with up to 5-6 targets can be detected simultaneously in the same well/reaction. This master mix was engineered with an enzyme multiple hot-start control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme Multiplex qPCR Probe Master Mix (2x), including buffer chemistry and incorporation of highly robust engineered enzymes mixed in an optimized proportion specifically designed for the highest performance. This master mix was specifically developed for probe-detection technology.
| Shipping Conditions | Room Temperature | Storage Conditions | -85 °C to -15 °C | Application | Multiplex Real-time qPCR | Starting Material | DNA | Features | Multiple Hot-start, Multiplex, Ultra-sensitive | Detection Method | Probe Based | Reference Dye | No ROX | Format | Liquid, Mastermix | Product Category | Real-Time PCR | Compatibility | Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | Concentration | 2x | Protocol time | Approx. 45 min. | Hot Start | Yes |
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– NZYSupreme Multiplex qPCR Probe Master Mix (2x)
| Shipping Conditions | Room Temperature | Storage Conditions | -85 °C to -15 °C | Application | Multiplex Real-time qPCR | Starting Material | DNA | Features | Multiple Hot-start, Multiplex, Ultra-sensitive | Detection Method | Probe Based | Reference Dye | No ROX | Format | Liquid, Mastermix | Product Category | Real-Time PCR | Compatibility | Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | Concentration | 2x | Protocol time | Approx. 45 min. | Hot Start | Yes |
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The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens.
Garrido-Maestu A, Lamas A, Tomás Fornés D, Prado Rodríguez M.
Methods in Molecular Biology (Springer Nature), 2024.
| Shipping Conditions | Room Temperature | Storage Conditions | -85 °C to -15 °C | Application | Multiplex Real-time qPCR | Starting Material | DNA | Features | Multiple Hot-start, Multiplex, Ultra-sensitive | Detection Method | Probe Based | Reference Dye | No ROX | Format | Liquid, Mastermix | Product Category | Real-Time PCR | Compatibility | Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | Concentration | 2x | Protocol time | Approx. 45 min. | Hot Start | Yes |
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| Shipping Conditions | Room Temperature | Storage Conditions | -85 °C to -15 °C | Application | Multiplex Real-time qPCR | Starting Material | DNA | Features | Multiple Hot-start, Multiplex, Ultra-sensitive | Detection Method | Probe Based | Reference Dye | No ROX | Format | Liquid, Mastermix | Product Category | Real-Time PCR | Compatibility | Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | Concentration | 2x | Protocol time | Approx. 45 min. | Hot Start | Yes |
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SDS
Safety Data Sheets
CoA
Certificate of Analysis
Manuals
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