NZYTrace qPCR Green Master Mix 2x (with UDG), ROX

NZYTrace qPCR Green Master Mix 2x (with UDG), ROX is an optimized, highly efficient reaction mixture for real-time PCR using a DNA-intercalating green dye. It combines a high-performance hot-start-like Taq DNA polymerase with advanced buffer chemistry and PCR enhancers to deliver ultra-sensitive, highly reproducible amplification in fast qPCR protocols.

The master mix features a distinct blue color and includes a powerful 40x concentrated NZY Yellow Template Buffer for superior results. When the yellow buffer is pre-mixed with the DNA (or cDNA) template and subsequently added to the blue master mix, it produces an immediate green reaction. This provides a visual confirmation of pipetting accuracy and plate layout, which is especially important for high-throughput or low-copy-number assays. NZYTrace qPCR Green Master Mix incorporates dUTP together with a thermolabile uracil-DNA glycosylase (UDG) to efficiently remove carryover contamination from uracil-containing amplicons generated in previous runs, helping to prevent false positives in demanding routine workflows. The presence of the passive reference dye ROX enables robust well-to-well normalization of non-PCR-related fluorescence fluctuations, increasing confidence in data analysis on ROX-dependent and ROX-compatible instruments.

NZYTrace qPCR Green Master Mix (with UDG), ROX is a ready-to-use 2X reaction mix containing a green DNA intercalating dye, a ROX passive reference dye, a balanced blend of dNTPs including dUTP and dTTP, optimized stabilizers and PCR enhancers. The mix requires only the addition of primers, DNA template and nuclease-free water. The optional NZY Yellow Template Buffer 40x may be used for visual verification of correct pipetting without affecting qPCR performance.

NZYTrace qPCR Green Master Mix 2x (with UDG), ROX is ideal for gene expression studies, pathogen and biomarker detection, and the reliable amplification of low-copy or otherwise challenging DNA targets. Its dual-color tracking, built-in UDG carryover control and ROX normalization make it particularly suited to high-throughput and routine screening workflows. Ideal for assay development and use across different real-time PCR instruments, while fully compatible with post-run melt curve analysis to confirm amplicon specificity.