Notification Bar Icon

Unlock 5% OFF: Shop online now and save on every purchase! Limited time offer.

Unlock 5% OFF: Shop online now and save on every purchase! Limited time offer.Learn more

Learn more
Only %1 left
SKU

NcoI

Storage Conditions:
-30 °C to -15 °C
NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by Dam methylation, Dcm methylation or CpG methylation.
Availability
Ready to Ship
Dry Ice
From €35.00
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences.

NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by Dam methylation, Dcm methylation and CpG methylation. The optimum reaction temperature is at 37 °C.


Concentration: 10000 U/mL

Reaction Buffer: The enzyme is provided with two buffers: a specific buffe – NZYBuffer B, optimized for routine single-enzyme digestion, and NZYBuffer U, a universal buffer suitable for multi-enzyme digestions and ready for direct agarose gel loading.
This dual-buffer system offers convenience and flexibility, ensuring maximal specificity and allowing customers to choose the most appropriate buffer for their experimental setup. For single-enzyme digestions using NZYBuffer B, NZYBuffer U can also be utilized as a loading buffer at a 1x concentration.

Conventional NZYTech Restriction Enzymes: Activity Guide
Activity Guide for Restriction Enzymes Page 1 Activity Guide for Restriction Enzymes Page 2
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers Bsp19I
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min.

  • NcoI (500 U)

  • 10x NZYBuffer B

  • 10x NZYBuffer U

More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers Bsp19I
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min.
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers Bsp19I
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min.

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

Related Products