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SalI
SalI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the SalI gene from Streptomyces albus G. SalI activity is blocked by CpG methylation. NZYtech’s SalI is an enzyme that acts at 37 °C.
Availability
Out of stock
Dry Ice
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences.
SalI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the SalI gene from Streptomyces albus G. SalI activity is blocked by CpG methylation. The optimum reaction temperature is at 37 °C.
Concentration: 10000 U/mL
Reaction Buffer: NZYtech provides SalI with a specific buffer, NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYtech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (20)%
NZYBuffer C: <20%
NZYBuffer U: 100%
() weak star activity is detected
Minimize exposure of enzyme to temperatures higher than -20 °C.
Concentration: 10000 U/mL
Reaction Buffer: NZYtech provides SalI with a specific buffer, NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYtech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (20)%
NZYBuffer C: <20%
NZYBuffer U: 100%
() weak star activity is detected
Minimize exposure of enzyme to temperatures higher than -20 °C.
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -30 °C to -15 °C |
| Application | DNA digestion |
| Features | Standard |
| Format | Liquid |
| Incubation Temperature | 37 °C |
| Product Category | Restriction Enzymes |
| Heat Inactivation | 65 °C for 20 minutes |
| Protocol time | Approx. 60 min |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -30 °C to -15 °C |
| Application | DNA digestion |
| Features | Standard |
| Format | Liquid |
| Incubation Temperature | 37 °C |
| Product Category | Restriction Enzymes |
| Heat Inactivation | 65 °C for 20 minutes |
| Protocol time | Approx. 60 min |
CoA
Certificate of Analysis
