Firefly Luciferase mRNA

Firefly Luciferase mRNA is a ready-to-use messenger RNA (mRNA) product encoding firefly luciferase, an enzyme derived from Photinus pyralis that produces a bioluminescent signal with a peak emission of approximately 560 nm via oxidation of the substrate D-luciferin. The product is commonly used in molecular biology as a reporter molecule for quantitative analysis of mRNA translation efficiency, bioluminescence imaging (BLI), and high-throughput screening of delivery systems and formulations. The transcript is designed for expression in eukaryotic cells. It contains the nucleic acid sequence encoding the 550 amino acid Firefly Luciferase protein sequence, together with non-coding regulatory elements typically required for efficient translation and stability (5′ untranslated region, 3′ untranslated region, and a 3′ poly(A) tail). The mRNA contains a 5′ Cap 1 structure, and all uridine residues are fully substituted with N1-methyl-pseudouridine (m¹Ψ) to support robust protein expression and to reduce innate immune sensing relative to unmodified mRNA. The mRNA is produced enzymatically by in vitro transcription from a DNA template, followed by capping, polyadenylation, and purification. Promoter sequences used to drive in vitro transcription from the DNA template are not part of the final mRNA product. For research use only (RUO). Not for diagnostic or therapeutic use, and not for human or animal administration.