mCherry mRNA

mCherry mRNA is a ready-to-use messenger RNA (mRNA) product encoding the red fluorescent protein mCherry, which exhibits an excitation maximum at 587 nm and an emission maximum at 610 nm. The product is commonly used in combination with other reporter molecules for dual-color or multi-color imaging experiments, co-transfection studies, comparative expression analysis, and tracking of protein localization in complex cellular environments. Its high photostability makes it well suited for long-term imaging applications. The transcript is designed for expression in eukaryotic cells. It contains the nucleic acid sequence encoding the 236 amino acid mCherry protein sequence, together with non-coding regulatory elements typically required for efficient translation and stability (5′ untranslated region, 3′ untranslated region, and a 3′ poly(A) tail). The mRNA contains a 5′ Cap 1 structure, and all uridine residues are fully substituted with N1-methyl-pseudouridine (m¹Ψ) to support robust protein expression and to reduce innate immune sensing relative to unmodified mRNA. The mRNA is produced enzymatically by in vitro transcription from a DNA template, followed by capping, polyadenylation, and purification. Promoter sequences used to drive in vitro transcription from the DNA template are not part of the final mRNA product. For research use only (RUO). Not for diagnostic or therapeutic use, and not for human or animal administration.