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T4 DNA Ligase
T4 DNA Ligase is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer, which includes ATP.
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Dry Ice
T4 DNA Ligase is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 4× Reaction Buffer, which includes ATP. T4 DNA Ligase catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. It repairs single-strand nicks in duplex DNA and will join both blunt and cohesive-end restriction fragments of duplex DNA or RNA. The enzyme requires ATP as cofactor.
Applications:
– Cloning of both blunt- and sticky (cohesive)-end restriction fragments
– Cloning of PCR products
– Joining linkers and adapters to DNA
– Nick repair
– Self-circularization of linear DNA
Applications:
– Cloning of both blunt- and sticky (cohesive)-end restriction fragments
– Cloning of PCR products
– Joining linkers and adapters to DNA
– Nick repair
– Self-circularization of linear DNA
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | Blunt-end ligation, Sticky-end ligation |
| Product Category | Ligases |
| Reaction Temperature | 16 - 20 ⁰C |
| Heat Inactivation | 65 °C |
| Concentration | 5 U/μL |
– T4 DNA Ligase (5 U/μL)
– Reaction buffer (4x)
– Reaction buffer (4x)
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | Blunt-end ligation, Sticky-end ligation |
| Product Category | Ligases |
| Reaction Temperature | 16 - 20 ⁰C |
| Heat Inactivation | 65 °C |
| Concentration | 5 U/μL |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | Blunt-end ligation, Sticky-end ligation |
| Product Category | Ligases |
| Reaction Temperature | 16 - 20 ⁰C |
| Heat Inactivation | 65 °C |
| Concentration | 5 U/μL |
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Certificate of Analysis
