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NZYMiniprep

Storage Conditions:
15 °C to 25 °C
NZYMiniprep kit allows for rapid preparation of small-scale highly pure plasmid DNA from E. coli strains.
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From €69.00
NZYMiniprep kits are designed for the rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. NZYTech´s miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt concentrations. The plasmid DNA is selectively adsorbed in the silica gel-based NZYTech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. NZYMiniprep kit includes an additional washing buffer (AY) which is strongly recommended for the complete removal of high levels of endonucleases. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 10 mL of E. coli cultures and double the volumes of Buffers A1, A2 and A3.
More Information
Shipping Conditions Room Temperature
Storage Conditions 15 °C to 25 °C
Application In Vitro transcription, Cloning, PCR, Sequencing, Transformation
Starting Material E. coli culture
Scale Miniprep
Features Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number
Format Column
Product Category DNA Purification, Plasmid DNA Purification
Isolation Technology Silica membrane
Protocol time Approx. 30 min.
– Buffer A1
– Buffer A2
– Buffer A3
– Buffer AY
– Buffer A4 (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
More Information
Shipping Conditions Room Temperature
Storage Conditions 15 °C to 25 °C
Application In Vitro transcription, Cloning, PCR, Sequencing, Transformation
Starting Material E. coli culture
Scale Miniprep
Features Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number
Format Column
Product Category DNA Purification, Plasmid DNA Purification
Isolation Technology Silica membrane
Protocol time Approx. 30 min.
Isolation of low-copy and high-copy number plasmid DNA (in duplicate) using NZYMiniprep or a competitor plasmid purification kit. Lane M: NZYDNA Ladder III.

Protocol overview:
More Information
Shipping Conditions Room Temperature
Storage Conditions 15 °C to 25 °C
Application In Vitro transcription, Cloning, PCR, Sequencing, Transformation
Starting Material E. coli culture
Scale Miniprep
Features Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number
Format Column
Product Category DNA Purification, Plasmid DNA Purification
Isolation Technology Silica membrane
Protocol time Approx. 30 min.
More Information
Shipping Conditions Room Temperature
Storage Conditions 15 °C to 25 °C
Application In Vitro transcription, Cloning, PCR, Sequencing, Transformation
Starting Material E. coli culture
Scale Miniprep
Features Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number
Format Column
Product Category DNA Purification, Plasmid DNA Purification
Isolation Technology Silica membrane
Protocol time Approx. 30 min.

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

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