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NZYMiniprep
NZYMiniprep kit allows for rapid preparation of small-scale highly pure plasmid DNA from E. coli strains.
NZYMiniprep kits are designed for the rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. NZYtech´s miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt concentrations. The plasmid DNA is selectively adsorbed in the silica gel-based NZYtech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. NZYMiniprep kit includes an additional washing buffer (AY) which is strongly recommended for the complete removal of high levels of endonucleases. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 10 mL of E. coli cultures and double the volumes of Buffers A1, A2 and A3.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
– Buffer A1
– Buffer A2
– Buffer A3
– Buffer AY
– Buffer A4 (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
– Buffer A2
– Buffer A3
– Buffer AY
– Buffer A4 (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
Isolation of low-copy and high-copy number plasmid DNA (in duplicate) using NZYMiniprep or a competitor plasmid purification kit. Lane M: NZYDNA Ladder III.
Protocol overview:
Protocol overview:
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
CoA
Certificate of Analysis
