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NZY-A PCR cloning kit

Storage Conditions:
Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
NZY-A PCR cloning kit was designed to allow the direct cloning of PCR products with 3´-A overhangs, which result from amplifications using non-proofreading DNA polymerases.
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Dry Ice
From €125.00
NZY-A PCR cloning kit was designed to allow the direct cloning of PCR products with 3´-A overhangs, which result from amplifications using non-proofreading DNA polymerases such as NZYTaq II DNA polymerase. The cloning vector was prepared by cutting NZYtech´s pNZY28 with EcoR V and adding a 3´ terminal thymidine at both ends. These single 3´-T overhangs improve the efficiency of ligation of a PCR product into the plasmid by preventing re-circularization of the vector and providing compatible overhangs for PCR products generated by non-proofreading thermostable polymerases. Vector pNZY28 contains multiple restriction sites within the multiple cloning region. However, vector digestion with EcoR I or BamH I allows the release of the PCR product since the vector cloning region is flanked by recognition sites of both enzymes. NZY-A PCR cloning system was developed to allow a rapid ligation (1 hour) between the vector and the PCR fragment.
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
Application TA Cloning
Features Blue/White screening
Product Category DNA Cloning & Mutagenesis
Protocol time Approx. 60 min
– NZY-A Buffer (2x)
– pNZY28-A vector (50 ng/µL)
– T4 DNA Ligase (2 U/µL)
– NZY-A positive control insert (50 ng/µL)
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)

(*) only provided in MB05301 kit
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
Application TA Cloning
Features Blue/White screening
Product Category DNA Cloning & Mutagenesis
Protocol time Approx. 60 min
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85 °C to -65 °C
–Other components: -30 °C to -15 °C
Application TA Cloning
Features Blue/White screening
Product Category DNA Cloning & Mutagenesis
Protocol time Approx. 60 min

MSDS

Material Safety Data Sheets

CoA

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