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NZY Reverse Transcriptase
NZY Reverse Transcriptase synthesizes cDNA strand in the presence of a primer using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50°C).
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Ready to Ship
Blue Ice / Ice Packs
NZY Reverse Transcriptase is a modified recombinant form of the Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase purified from Escherichia coli. The enzyme has been modified in order to promote stability. NZY Reverse Transcriptase synthesizes the complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template in a wide range of temperatures (37-50°C). High reaction temperatures are beneficial to improve specificity and to allow synthesis of cDNA from complex templates, including those with high GC-content or with high degree of secondary structure. The enzyme lacks 3´→5´ exonuclease activity and has no RNase H activity, enabling improved synthesis of full-length cDNA even for long mRNA, using random priming. Thus, NZY Reverse Transcriptase gives high yields of cDNA up to 7 kb. NZY Reverse Transcriptase can be used in first-strand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and analysis of RNA by primer extension.
| Shipping Conditions | Blue Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Application | cDNA synthesis, RT-PCR, RT-qPCR |
| Features | Fast, High yield, Thermostable |
| Format | Enzyme/Protein, Liquid |
| Product Category | cDNA Synthesis |
| Reaction Temperature | 50 °C |
| Heat Inactivation | 85 °C |
| Protocol time | Approx. 60 min. |
– NZY Reverse Transcriptase (200 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
| Shipping Conditions | Blue Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Application | cDNA synthesis, RT-PCR, RT-qPCR |
| Features | Fast, High yield, Thermostable |
| Format | Enzyme/Protein, Liquid |
| Product Category | cDNA Synthesis |
| Reaction Temperature | 50 °C |
| Heat Inactivation | 85 °C |
| Protocol time | Approx. 60 min. |
Genetic diversity of norovirus in children under 5 years of age with acute gastroenteritis from Angola
Esteves A, Nordgren J, Tavares C, Fortes F, Dimbu R, Saraiva N, Istrate C
Epidemiology & Infection, 2018
The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells
Cipriano M, Correia JC, Camões SP, Oliveira NG, Cruz P, Cruz H, Castro M, Ruas JL, Santos JM, Miranda JP
Archives of Toxicology, 2017
Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal
Pereira A, Figueira L, Nunes M, Esteves A, Cotão AJ, Vieira ML, Maia C, Campino L, Parreira R
Ticks and Tick-borne Diseases, 2017
Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction
Gasparinho C, Piedade J, Mirante MC, Mendes C, Mayer C, Vaz Nery S, Brito M, Istrate C
PLoS One, 2017
Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro
Cipriano M, Freyer N, Knöspel F, Oliveira NG, Barcia R, Cruz PE, Cruz H, Castro M, Santos JM, Zeilinger K, Miranda JP
Archives of Toxicology, 2017
Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction
Esteves A, Nordgren J, Pereira J, Fortes F, Dimbu R, Saraiva N, Mendes C, Istrate C
Journal of Medical Virology, 2016
Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10
Madureira PA, Bharadwaj AG., Bydoun M, Garant K, O’Connell P, Lee P, Waisman DM
Oncotarget, 2016
Enhancing glioblastoma cell sensitivity to chemotherapeutics: A strategy involving survivin gene silencing mediated by gemini surfactant-based complexes
Cruz RQ, Morais CM, Cardoso AM, Silva SG, Vale ML, Marques EF, Pedroso de Lima MC, Jurado AS
European Journal of Pharmaceutics and Biopharmaceutics, 2016
Histopathological and in vivo evidence of regucalcin as a protective molecule in mammary gland carcinogenesis
Marques R, Vaz CV, Maia CJ, Gomes M, Gama A, Alves G, Santos CR, Schmitt F, Socorro S
Experimental Cell Research, 2015
Aging-associated changes in oxidative stress, cell proliferation, and apoptosis are prevented in the prostate of transgenic rats overexpressing regucalcin
Vaz CV, Marques, R, Maia, CJ, Socorro S.
Translational Research, 2015
5a-Dihydrotestosterone regulates the expression of L-type calcium
Marques R, Peres CG, Vaz CV, Gomes IM, Figueira MI, Cairrão E, Verde I, Maia M, Socorro S
Medical Oncology, 2015
Gemini surfactants mediate efficient mitochondrial gene delivery and expression
Cardoso AM, Morais CM, Cruz AR, Cardoso AL, Silva SG, do Vale ML, Marques EF, Pedroso de Lima MC, Jurado AS.
Mol. Pharmaceutics, 2015
Molecular and functional characterization of an invertase secreted by Ashbya gossypii
Aguiar TQ, Dinis C, Magalhães F, Oliveira C, Wiebe MG, Penttila M, Domingues L
Molecular Biotechnology, 2014
Gilthead seabream (Sparus aurata) immune responses are modulated after feeding with purified antinutrients
Costas B, Couto A, Azeredo R, Machado M, Krogdahl A, Oliva-Teles A
Fish & Shellfish Immunology, 2014
Esteves A, Nordgren J, Tavares C, Fortes F, Dimbu R, Saraiva N, Istrate C
Epidemiology & Infection, 2018
The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells
Cipriano M, Correia JC, Camões SP, Oliveira NG, Cruz P, Cruz H, Castro M, Ruas JL, Santos JM, Miranda JP
Archives of Toxicology, 2017
Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal
Pereira A, Figueira L, Nunes M, Esteves A, Cotão AJ, Vieira ML, Maia C, Campino L, Parreira R
Ticks and Tick-borne Diseases, 2017
Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction
Gasparinho C, Piedade J, Mirante MC, Mendes C, Mayer C, Vaz Nery S, Brito M, Istrate C
PLoS One, 2017
Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro
Cipriano M, Freyer N, Knöspel F, Oliveira NG, Barcia R, Cruz PE, Cruz H, Castro M, Santos JM, Zeilinger K, Miranda JP
Archives of Toxicology, 2017
Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction
Esteves A, Nordgren J, Pereira J, Fortes F, Dimbu R, Saraiva N, Mendes C, Istrate C
Journal of Medical Virology, 2016
Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10
Madureira PA, Bharadwaj AG., Bydoun M, Garant K, O’Connell P, Lee P, Waisman DM
Oncotarget, 2016
Enhancing glioblastoma cell sensitivity to chemotherapeutics: A strategy involving survivin gene silencing mediated by gemini surfactant-based complexes
Cruz RQ, Morais CM, Cardoso AM, Silva SG, Vale ML, Marques EF, Pedroso de Lima MC, Jurado AS
European Journal of Pharmaceutics and Biopharmaceutics, 2016
Histopathological and in vivo evidence of regucalcin as a protective molecule in mammary gland carcinogenesis
Marques R, Vaz CV, Maia CJ, Gomes M, Gama A, Alves G, Santos CR, Schmitt F, Socorro S
Experimental Cell Research, 2015
Aging-associated changes in oxidative stress, cell proliferation, and apoptosis are prevented in the prostate of transgenic rats overexpressing regucalcin
Vaz CV, Marques, R, Maia, CJ, Socorro S.
Translational Research, 2015
5a-Dihydrotestosterone regulates the expression of L-type calcium
Marques R, Peres CG, Vaz CV, Gomes IM, Figueira MI, Cairrão E, Verde I, Maia M, Socorro S
Medical Oncology, 2015
Gemini surfactants mediate efficient mitochondrial gene delivery and expression
Cardoso AM, Morais CM, Cruz AR, Cardoso AL, Silva SG, do Vale ML, Marques EF, Pedroso de Lima MC, Jurado AS.
Mol. Pharmaceutics, 2015
Molecular and functional characterization of an invertase secreted by Ashbya gossypii
Aguiar TQ, Dinis C, Magalhães F, Oliveira C, Wiebe MG, Penttila M, Domingues L
Molecular Biotechnology, 2014
Gilthead seabream (Sparus aurata) immune responses are modulated after feeding with purified antinutrients
Costas B, Couto A, Azeredo R, Machado M, Krogdahl A, Oliva-Teles A
Fish & Shellfish Immunology, 2014
| Shipping Conditions | Blue Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Application | cDNA synthesis, RT-PCR, RT-qPCR |
| Features | Fast, High yield, Thermostable |
| Format | Enzyme/Protein, Liquid |
| Product Category | cDNA Synthesis |
| Reaction Temperature | 50 °C |
| Heat Inactivation | 85 °C |
| Protocol time | Approx. 60 min. |
| Shipping Conditions | Blue Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Application | cDNA synthesis, RT-PCR, RT-qPCR |
| Features | Fast, High yield, Thermostable |
| Format | Enzyme/Protein, Liquid |
| Product Category | cDNA Synthesis |
| Reaction Temperature | 50 °C |
| Heat Inactivation | 85 °C |
| Protocol time | Approx. 60 min. |
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Certificate of Analysis
