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NZY Total RNA Isolation kit
NZY Total RNA Isolation kit is designed for the easy purification of total RNA of highest integrity from a variety of sources.
NZY Total RNA Isolation kit is designed for the easy purification of total RNA of highest integrity (longer than 200 bases) from a variety of sources such as animal tissues, bacteria cells and cell cultures. This method uses a denaturing lysis buffer containing guanidine thiocyanate, which inactivates cellular RNases, to ensure the recovery of intact RNA molecules. Ethanol is added to provide selective binding of total RNA into the silica membrane column and impurities are efficiently washed away. To prevent the contamination with DNA, a DNase I solution is directly added onto the silica membrane of the binding column. High-quality RNA is then eluted in RNase-free water.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Starting Material | Animal Tissue, Cultured Cells, Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | DNA removal, Manual | Format | Column | Product Category | RNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
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– Buffer NR
– Buffer NI
– Buffer NRW1
– Buffer NRW2 (concentrated)
– RNase-free water
– Digestion buffer
– DNase I (lyophilized)
– NZYSpin Homogenization Columns (purple rings)
– NZYSpin Binding Columns (blue rings)
– Collection tubes (1.5 mL and 2 mL)
– Buffer NI
– Buffer NRW1
– Buffer NRW2 (concentrated)
– RNase-free water
– Digestion buffer
– DNase I (lyophilized)
– NZYSpin Homogenization Columns (purple rings)
– NZYSpin Binding Columns (blue rings)
– Collection tubes (1.5 mL and 2 mL)
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Starting Material | Animal Tissue, Cultured Cells, Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | DNA removal, Manual | Format | Column | Product Category | RNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
Parasite and vector circadian clocks mediate efficient malaria transmission.
Bento I, Parrington B, Pascual R, Goldberg A, Liu H, Borrmann H, Zelle M, Coburn N, Takahashi J, Elias J, Mota M, Rijo-Ferreira, F.
Nature Microbiology, 2025.
A plasmid-chromosome crosstalk in multidrug resistant enterobacteria.
Toribio-Celestino L, Calvo-Villamañán A, Herencias C, Alonso-del Valle A, Sastre-Dominguez J, Quesada S, Mazel D, Rocha EPC, Fernández-Calvet A, San Millán Á.
Nature Communications, 2024.
Cytokine profiling and transcriptomics in mononuclear cells define immune variants in Meniere Disease.
Flook M, Rojano E, Gallego-Martinez A, Escalera-Balsera A, Perez-Carpena P, del Carmen Moleon M, Gonzalez-Aguuado R, Rivero de Jesus V, Domínguez-Durán E, Frejo L, Ranea J, Lopez-Escamez J A.
Genes & Immunity (Nature), 2024.
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2.
Alonso-Gil D, Cuadrado A, Giménez-Llorente D, Rodríguez-Corsino M, Losada A.
Nature Communications, 2023.
Host lung microbiota promotes malaria-associated acute respiratory distress syndrome.
Mukherjee D, Ferreira Chora A, Lone J, Ramiro R, Blamkenhaus B, Serre K, Ramirez M, Gordo I, Veldhoen M, Varga-Weisz P, Mota M.
Nature Communications, 2022.
β-Cryptoxanthin Inhibits Angiogenesis in Human Umbilical Vein Endothelial Cells Through Retinoic Acid Receptor.
Quesada-Gómez JM, Santiago-Mora R, Durán-Prado M, Dorado G, Pereira-Caro G, Moreno-Rojas JM, Casado-Díaz A.
Mol Nutr Food Res, 2018.
Estrogen Modulates Glycerol Permeability in Sertoli Cells through Downregulation of Aquaporin-9.
Bernardino RL, Carrageta DF, Silva AM, Calamita G, Alves MG, Soveral G Oliveira PF.
Cells, 2018.
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT1444 are secreted into the lumen of the inclusion.
da Cunha M, Pais S V, Bugalhão J N, Mota L J.
PLoS One, 2017.
The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells.
Cipriano M Correia JC, Camões SP, Oliveira NG, Cruz P, Cruz H, Castro M, Ruas JL, Santos JM, Miranda JP.
Arch Toxicol, 2017.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Starting Material | Animal Tissue, Cultured Cells, Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | DNA removal, Manual | Format | Column | Product Category | RNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Starting Material | Animal Tissue, Cultured Cells, Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | DNA removal, Manual | Format | Column | Product Category | RNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 30 min. |
|---|
SDS
Safety Data Sheets
CoA
Certificate of Analysis
