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NZY Tissue gDNA Isolation kit
NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample using silica-based spin column.
NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample sources including animal cells and tissues, Gram-positive and Gram-negative bacteria, mouse tails, yeast, forensic samples and clinical samples.
The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | Genotyping, PCR, qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Body fluids, Buccal swabs, Forensic samples, Gram-negative bacteria, Gram-positive bacteria, Mouse tails, Paraffin embedded tissue, Plasma, Serum, Stool, Whole blood, Yeast |
| Scale | Miniprep |
| Features | Manual |
| Format | Column |
| Product Category | DNA Purification, Genomic DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
– Buffer NT1
– Buffer NL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– NZYSpin Tissue columns (light green ring)
– Collection tubes (2 mL)
– Buffer NL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– NZYSpin Tissue columns (light green ring)
– Collection tubes (2 mL)
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | Genotyping, PCR, qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Body fluids, Buccal swabs, Forensic samples, Gram-negative bacteria, Gram-positive bacteria, Mouse tails, Paraffin embedded tissue, Plasma, Serum, Stool, Whole blood, Yeast |
| Scale | Miniprep |
| Features | Manual |
| Format | Column |
| Product Category | DNA Purification, Genomic DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
The Chlamydia trachomatis type III secretion substrates CT142, CT143 and CT144 are secreted into the lumen of the inclusion
da Cunha M, Pais SV, Bugalhão JN, Mota LJ.
PLoS One, 2017
Interaction studies between periplasmic cytochromes provide insights into extracellular electron transfer pathways of Geobacter sufurreducens
Fernandes AP, Nunes TC, Paquete CM, Salgueiro CA.
Biochem J , 2017
Does DNA methylation regulate metamorphosis? The case of the sea lamprey (Petromyzon marinus) as an example
Covelo-Soto L, Saura M, Morán P.
Comp Biochem Physiol B Biochem Mol Biol, 2015
da Cunha M, Pais SV, Bugalhão JN, Mota LJ.
PLoS One, 2017
Interaction studies between periplasmic cytochromes provide insights into extracellular electron transfer pathways of Geobacter sufurreducens
Fernandes AP, Nunes TC, Paquete CM, Salgueiro CA.
Biochem J , 2017
Does DNA methylation regulate metamorphosis? The case of the sea lamprey (Petromyzon marinus) as an example
Covelo-Soto L, Saura M, Morán P.
Comp Biochem Physiol B Biochem Mol Biol, 2015
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | Genotyping, PCR, qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Body fluids, Buccal swabs, Forensic samples, Gram-negative bacteria, Gram-positive bacteria, Mouse tails, Paraffin embedded tissue, Plasma, Serum, Stool, Whole blood, Yeast |
| Scale | Miniprep |
| Features | Manual |
| Format | Column |
| Product Category | DNA Purification, Genomic DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | Genotyping, PCR, qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Body fluids, Buccal swabs, Forensic samples, Gram-negative bacteria, Gram-positive bacteria, Mouse tails, Paraffin embedded tissue, Plasma, Serum, Stool, Whole blood, Yeast |
| Scale | Miniprep |
| Features | Manual |
| Format | Column |
| Product Category | DNA Purification, Genomic DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 30 min. |
CoA
Certificate of Analysis
