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NZYProof DNA polymerase
NZYProof DNA polymerase is a recombinant DNA polymerase that presents high fidelity and displays great performance in the majority of PCR applications.
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Blue Ice / Ice Packs
NZYProof DNA polymerase is a recombinant DNA polymerase that presents high fidelity and displays great performance in the majority of PCR applications. NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading capacity which enables the polymerase to amplify DNA with increased accuracy. The enzyme is highly efficient in the amplification of longer (≤10 kb) PCR products and site-directed mutagenesis. In addition, it the recommended polymerase for routine cloning that requires precision. The error rate of NZYProof DNA polymerase is similar to that of Pfu and Kod DNA polymerases and significantly lower than the error rate of Taq DNA polymerases. NZYProof DNA polymerase generates bluntended PCR products that are suitable for cloning with NZYtech’s NZY-blunt PCR cloning kit (MB121). NZYProof DNA Polymerase is supplied with a 10x Reaction Buffer, which contains Mg2+ at an optimal concentration for standard PCR, and a 5x Stabilizer Solution, which maximizes yields especially when using lower template concentrations.
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | Blunt-end Cloning, Routine PCR, Site-directed mutagenesis |
Features | High-fidelity |
Product Length | 0-10 kb |
Format | Enzyme/Protein |
Product Category | End-Point PCR |
Concentration | 2.5 U/μL |
Hot Start | No |
– NZYProof DNA polymerase (2.5 U/μL)
– Reaction buffer (10x)
– Stabilizer Solution (5x)
– Reaction buffer (10x)
– Stabilizer Solution (5x)
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | Blunt-end Cloning, Routine PCR, Site-directed mutagenesis |
Features | High-fidelity |
Product Length | 0-10 kb |
Format | Enzyme/Protein |
Product Category | End-Point PCR |
Concentration | 2.5 U/μL |
Hot Start | No |
Turning a Hyperthermostable Metallo-Oxidase into a Laccase by Directed Evolution
Brissos V, Ferreira M, Grass G, Martins LO
ACS Catal, 2015
Improving kinetic or thermodynamic stability of an azoreductase by directed evolution
Brissos V, Gonçalves N, Melo EP, Martins LO
PLoS One, 2014
Brissos V, Ferreira M, Grass G, Martins LO
ACS Catal, 2015
Improving kinetic or thermodynamic stability of an azoreductase by directed evolution
Brissos V, Gonçalves N, Melo EP, Martins LO
PLoS One, 2014
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | Blunt-end Cloning, Routine PCR, Site-directed mutagenesis |
Features | High-fidelity |
Product Length | 0-10 kb |
Format | Enzyme/Protein |
Product Category | End-Point PCR |
Concentration | 2.5 U/μL |
Hot Start | No |
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | Blunt-end Cloning, Routine PCR, Site-directed mutagenesis |
Features | High-fidelity |
Product Length | 0-10 kb |
Format | Enzyme/Protein |
Product Category | End-Point PCR |
Concentration | 2.5 U/μL |
Hot Start | No |
CoA
Certificate of Analysis