We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
SKU
MB21702
NZYMicrobial gDNA Isolation Kit
NZY Microbial gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of microbial samples.
NZY Microbial gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of microbial samples. This kit ensures cell wall lysis using mechanical disruption instead of enzymatic methods. Difficult to lyse microbial samples such as yeast, gram-positive bacteria and spores can be lysed using NZY Microbial gDNA Isolation kit. NZYSpin Microbial Bead Tubes replace enzymatic lysis and by mechanical disruption they can lyse strong complex cell wall structures. Bacterium and yeast such as Corynebacterium glutamicum, Saccharomyces cerevisiae, Bacillus subtilis and Escherichia coli are examples of microbial organisms whose DNA can be isolated using this kit. NZY Microbial gDNA Isolation kit is optimized to isolate 5-25 µg of DNA from up to 30 mg wet weight of microbial pellet, depending of the type of sample.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | Manual, Mechanical lysis (glass beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
– Buffer NML
– Buffer NMW1
– Buffer NMW2 (concentrate)
– Buffer NME
– Proteinase K (liquid)
– NZYSpin Microbial Bead Tubes
– NZYSpin Microbial Columns (light green rings)
– Collection tubes (2 mL)
– Buffer NMW1
– Buffer NMW2 (concentrate)
– Buffer NME
– Proteinase K (liquid)
– NZYSpin Microbial Bead Tubes
– NZYSpin Microbial Columns (light green rings)
– Collection tubes (2 mL)
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | Manual, Mechanical lysis (glass beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
Monitoring within-farm transmission dynamics of antimicrobial-resistant Campylobacter in dairy cattle using broth microdilution and long-read whole genome sequencing.
Ocejo M, Oporto B, Lavín J, Hurtado A.
Scientific Reports (Nature), 2023.
The car tank lid bacteriome: a reservoir of bacteria with potential in bioremediation of fuel.
Vidal-Verdú A, Gómez-Martínez D, Latorre-Pérez A, Peretó J, Porcar M.
npj Biofilms and Microbiomes (Nature Portfolio), 2022.
Whole genome-based characterisation of antimicrobial resistance and genetic diversity in Campylobacter jejuni and Campylobacter coli from ruminants.
Ocejo M, Oporto B, Lavín J, Hurtado A.
Scientific Reports (Nature), 2021.
Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes.
Lara-Moreno A, El-Sayed F, Cox C, Costa M, Carlier J.
Applied Water Science (Springer), 2024.
Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm.
Contente D, Ponce-Alonso M, Díaz-Formoso L, Araújo C, Peña N, Borrero J, Gómez-Sala B, del Campo R,Muñoz-Atienza E, Hernández P, Cintas L.
Microorganisms (MDPI), 2022.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | Manual, Mechanical lysis (glass beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
Protocol overview:
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | Manual, Mechanical lysis (glass beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Gram-negative bacteria, Gram-positive bacteria, Yeast | Scale | Miniprep | Features | Manual, Mechanical lysis (glass beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
SDS
Safety Data Sheets
CoA
Certificate of Analysis
