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SKU
MB21802
NZYSoil gDNA Isolation Kit
NZY Soil gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of soil microorganisms.
NZY Soil gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of soil microorganisms, such as bacteria, archaea, fungi, and algae in soil, sludge and sediment samples. This kit is suitable for samples from forest, bog, farmland, grassland and stool samples. The samples are mechanically disrupted using ceramic beads. Proteins and other PCR inhibitors in solution are precipitated with Buffer NSL3 followed by centrifugation with the ceramic beads. Any residual humic substances and other PCR inhibitors are efficiently removed in the washing steps. High yields with excellent purity from all types of samples are possible due to the combination of Buffer NSL1 and Buffer NSL2 with the additive NS enhancer. NZY Soil gDNA Isolation kit is optimized to isolate 2-10 µg of DNA from up to 500 mg of soil or sediment.
Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Soil, Stool | Scale | Miniprep | Features | Inhibitors removal (column), Manual, Mechanical lysis (ceramic beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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– Buffer NSL1
– Buffer NSL2
– Buffer NSL3
– NS Enhancer
– Buffer NSB
– Buffer NSW1
– Buffer NSW2 (concentrate)
– Buffer NSE
– NZYSpin Soil Bead Tubes
– NZYSpin Soil Inhibitor Removal Columns (red rings)
– NZYSpin Soil Columns (green rings)
– Collection Tubes (2 mL)
– Collection tubes (2 mL, lid)
– Buffer NSL2
– Buffer NSL3
– NS Enhancer
– Buffer NSB
– Buffer NSW1
– Buffer NSW2 (concentrate)
– Buffer NSE
– NZYSpin Soil Bead Tubes
– NZYSpin Soil Inhibitor Removal Columns (red rings)
– NZYSpin Soil Columns (green rings)
– Collection Tubes (2 mL)
– Collection tubes (2 mL, lid)
Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Soil, Stool | Scale | Miniprep | Features | Inhibitors removal (column), Manual, Mechanical lysis (ceramic beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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Protocol overview:
Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Soil, Stool | Scale | Miniprep | Features | Inhibitors removal (column), Manual, Mechanical lysis (ceramic beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | Genotyping, PCR, qPCR, Sequencing | Starting Material | Soil, Stool | Scale | Miniprep | Features | Inhibitors removal (column), Manual, Mechanical lysis (ceramic beads) | Format | Column | Product Category | DNA Purification, Genomic DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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CoA
Certificate of Analysis