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NZYSpeedy qPCR Probe Master Mix (2x), ROX

Storage Conditions:
-85 °C to -15 °C
NZYSpeedy qPCR Probe Master Mix (2x), ROX is an optimized mix developed for real-time PCR, specifically for probe-detection technology. The latest PCR enhancers and a polymerase with hot start-like activity guarantee sensitivity, reproducibility, and fast assays.
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Ready to Ship
Blue Ice / Ice Packs
From €136.00
NZYSpeedy qPCR Probe Master Mix (2x), ROX is an optimized and highly efficient reaction mixture developed for real-time PCR. This master mix enables fast and highly reproducible procedures on the most common real-time PCR apparatus. This kit was developed for probe-detection technology, including molecular beacons. The latest developments in PCR enhancers have been incorporated in this master mix, including buffer chemistry and a polymerase with hot start like activity. These combinations guarantee that NZYSpeedy qPCR Probe Master Mix, ROX delivers sensitivity coupled with highly reproducible and fast real-time PCR protocols. NZYSpeedy qPCR Probe Master Mix (2x), ROX is provided as a 2× reaction mixture containing all components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Fast, Hot-start
Detection Method Probe Based
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes
– NZYSpeedy qPCR Probe Master Mix (2x), ROX
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Fast, Hot-start
Detection Method Probe Based
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes

Fucose dependent rotavirus and norovirus require fucosidase activity for optimal replication.
Peña-Gil N, Santos-Ferreira N, Llanos-Villatoro S, Melero A, Cárcamo-Calvo R, López-Navarro S, Santiso-Bellón C, Buesa J, Monedero V, Yebra Maria J, Rocha-Pereira J, Gozalbo-Rovira R, Rodríguez-Díaz J.
npj Viruses (Nature Portfolio), 2025. 




Insights into histone deacetylase inhibitors-induced cell death in cancer cell lines.
Barberá V M, de Juan Romero C, Fuentes-Baile M, García-Morales P, Mallavia R, Martín-Orozco E, Mata-Balaguer T, Menéndez-Gutiérrez M P, Pérez-Valenciano E, Rodríguez-Lescure Á, Saceda M.
Biomedicines (MDPI), 2025. 




Microbiota Depletion Promotes Human Rotavirus Replication in an Adult Mouse Model.
Buesa J, Gozalbo-Rovira R, Monedero V, Muñoz C, Rubio-del-Campo A, Santiso-Bellón C, Vila-Vicent S, Yebra M J, Rodríguez-Díaz J.
Biomedicine & Pharmacotherapy (Elsevier), 2021. 

More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Fast, Hot-start
Detection Method Probe Based
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Fast, Hot-start
Detection Method Probe Based
Reference Dye ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility AB™ 7500, AB™ 7500 Fast, AB™ QuantStudio™ 12K, AB™ QuantStudio™ 3, AB™ QuantStudio™ 5, AB™ QuantStudio™ 6, AB™ QuantStudio™ 7, AB™ QuantStudio™ ViiA7™, Agilent AriaMx, Agilent Mx3000P™, Agilent Mx3005P™, Agilent Mx4000™, Fluidigm® Biomark™
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes

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