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SKU
MB40001

NZY First-Strand cDNA Synthesis Flexible Pack

Storage Conditions:
-85 °C to -15 °C
NZY First-Strand cDNA Synthesis Flexible Pack includes all the necessary components to synthesize first-strand cDNA from an RNA template. In addition, it offers the possibility to choose the primer to initiate the reaction.
Availability
Ready to Ship
Dry Ice
Unit Size
200 reactions
€224.00
NZY First-Strand cDNA Synthesis Flexible Pack is an optimized pack that includes the following components: the modified recombinant form of the Moloney Murine Leukemia Virus (MMuLV) Reverse Transcriptase (RNase H minus), which synthesizes the complementary DNA strand using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50 °C); an optimized Reverse Transcriptases buffer; the NZY Ribonuclease Inhibitor for RNA protection against degradation due to ribonuclease contamination; a dNTPs mix; random hexamers and oligo(dT)18 primers; and finally, an E. coli RNase H to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This flexible pack offers the possibility to choose the primer to initiate the reaction: you can choose either the primers provided in separate tubes, or a gene-specific primer. The pack is sufficient for up to 200 first-strand cDNA synthesis reactions and it is optimized for RT reactions over a wide range of total RNA concentrations from 10 pg to 5 μg. Downstream applications include real-time PCR, standard PCR and microarrays.
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Fast, Flexible primer selection, High yield, Thermostable
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 50 °C
Protocol time Approx. 60 min.
– NZY Reverse Transcriptase (200 U/μL)
– 10x Reaction buffer for Reverse Transcriptases
– NZY Ribonuclease Inhibitor (40 U/µL)
– dNTP Mix (10 mM each)
– Random hexamer mix (50 ng/µL)
– Oligo(dT)18 primer mix (50 μM)
– NZY RNase H (E. coli) (5 U/μL)
– DEPC-treated H2O
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Fast, Flexible primer selection, High yield, Thermostable
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 50 °C
Protocol time Approx. 60 min.
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Fast, Flexible primer selection, High yield, Thermostable
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 50 °C
Protocol time Approx. 60 min.

MSDS

Material Safety Data Sheets

CoA

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Please insert your lot number to get the available CoA

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