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NZYSupreme qPCR Probe Master Mix (2x)

Storage Conditions:
-85 °C to -15 °C
NZYSupreme qPCR Probe Master Mix (2x) is a high-performing, easy-to-use mixture for real-time PCR, specifically for probe-detection technology. It features a multiple hot-start polymerase control for maximum sensitivity.
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From €107.00
NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient reaction mixture developed for real-time PCR. This master mix was engineered with a multiple hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require this reference dye, we indicate a concentration range for your assay according to the table shown in the section “ROX reference dye” (ROX not provided). NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Probe Based
Reference Dye No ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes
– NZYSupreme qPCR Probe Master Mix (2x)
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Probe Based
Reference Dye No ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes

Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product.
Azinheiro S, Rodríguez-López P, Lozano-León A, Guedes H, Regal P, Franco CM, Cepeda A, Teixeira P, Melo LDR & Garrido-Maestu A.
Food Control (Elsevier), 2023. 




Molecular Detection of Plasmodium falciparum Infections Missed by Microscopy and HRP2-Based RDTs in the Dominican Republic.
Aquino E, Baptista V, Cabrera Federo C, Conte M, Cruz M, Cuevas F, Disla M, Freitas B, Gonçalves A.M, Miguel I, Osório N.S, Santos-Pereira, A, Tabata A, Veiga M.I.
Acta Tropica (Elsevier), 2025. 

More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Probe Based
Reference Dye No ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Starting Material DNA
Features Multiple Hot-start, Ultra-sensitive
Detection Method Probe Based
Reference Dye No ROX
Format Liquid, Mastermix
Product Category Real-Time PCR
Compatibility Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4™, Bio-Rad iCycler™ IQ, Bio-Rad iCycler™ IQ 4, Bio-Rad iCycler™ IQ 5, Bio-Rad iCycler™ MiniOpticon, Bio-Rad MyiQ™, Bio-Rad Opticon2™, Bio-Rad Opticon™, Bioer Gene Touch 96, Illumina Eco™, Roche LightCycler® 480, Roche LightCycler® 96, Roche LightCycler® Nano, Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q
Concentration 2x
Protocol time Approx. 45 min.
Hot Start Yes

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