NZY Uracil-DNA Glycosylase (UDG)

NZY Uracil-DNA Glycosylase is an engineered version of a psychrophilic Uracil-DNA Glycosylase (UDG) that catalyses the release of free uracil from uracil-containing DNA templates. The enzyme is optimized for removal of potentially carry-over contaminant uracil-containing LAMP or PCR products in LAMP or PCR/qPCR scenarios, respectively. NZY Uracil-DNA Glycosylase efficiently hydrolyses uracil from single-stranded or doublestranded DNA (although it is more active on ssDNA), but not from RNA. The enzyme excises uracil, thus creating alkali-sensitive abasic sites in the DNA. These abasic sites can be hydrolysed by heat and thus the contaminant dU-containing LAMP/PCR products from previous experiments can no longer serve as amplification templates. Since the enzyme is inactivated at temperatures of 55°C or higher, NZY Uracil-DNA Glycosylase is recommended for removal of carry-over contaminants exclusively in LAMP or PCR/qPCR reactions. Thus, NZY Uracil-DNA Glycosylase is not recommended for removal of carry-over contaminants from RTLAMP or RT-PCR/qPCR reactions that include a reversion transcription step at 45-55°C. At these temperatures NZY UracilDNA Glycosylase remains partially active and thus will degrade the newly generated uracil-containing cDNA chains.