Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack

Storage Conditions:

-85 °C to -15 °C

Provides all the required components to efficiently synthesize first-strand cDNA from an RNA template, offering flexible priming options.

Availability

Ready to Ship

Dry Ice

Details

Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack is a versatile kit designed for efficient, fast and reliable first-strand cDNA synthesis. It offers a comprehensive solution, including:

High-Performance Reverse Transcriptase: Supreme NZY Reverse Transcriptase 1 kU/µL, known for its stability, accuracy, and ability to synthesize full-length cDNA;
Optimized Buffer: 10x Reaction Buffer specifically formulated for the reverse transcriptase, ensuring optimal enzyme performance;
RNase Inhibitor: Supreme NZY Ribonuclease Inhibitor is included to protect RNA from degradation;
dNTPs: A balanced mix of dNTPs for complete cDNA synthesis;
Primers: Both random hexamers and oligo(dT) ₁₈ primers are provided for flexible priming options;
RNase H: For RNA degradation in cDNA-RNA hybrids, essential for downstream applications.

The pack is suitable for a wide range of RNA samples and total RNA concentrations, making it adaptable to various experimental needs. The produced cDNA can be used for various downstream applications, including real-time PCR, standard PCR, and microarrays. Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack offers a streamlined and efficient workflow for cDNA synthesis, providing researchers with a reliable and convenient tool for their molecular biology experiments.

More Information
Shipping Conditions	Blue Ice
Storage Conditions	-85 °C to -15 °C
Application	cDNA synthesis, RT-PCR, RT-qPCR
Features	Fast protocol, Flexible primer selection, High Accuracy and Processivity, Inhibitor resistant, Thermostable
Format	Kit, Liquid
Product Category	cDNA Synthesis
Reaction Temperature	30-70 °C
Heat Inactivation	85 °C
Protocol time	Approx. 5 min.

Components

Supreme NZY Reverse Transcriptase 1 kU/µL

Dilution Buffer for Supreme Reverse Transcriptase

Reaction Buffer 10x for Supreme NZY Reverse Transcriptase

Supreme NZY Ribonuclease Inhibitor

dNTP Mix (10mM each)

Oligo (dT)₁₈ primer mix

Random hexamer mix

NZY RNase H (E. coli)

DEPC-treated H₂O

More Information
Shipping Conditions	Blue Ice
Storage Conditions	-85 °C to -15 °C
Application	cDNA synthesis, RT-PCR, RT-qPCR
Features	Fast protocol, Flexible primer selection, High Accuracy and Processivity, Inhibitor resistant, Thermostable
Format	Kit, Liquid
Product Category	cDNA Synthesis
Reaction Temperature	30-70 °C
Heat Inactivation	85 °C
Protocol time	Approx. 5 min.

More Information
Shipping Conditions	Blue Ice
Storage Conditions	-85 °C to -15 °C
Application	cDNA synthesis, RT-PCR, RT-qPCR
Features	Fast protocol, Flexible primer selection, High Accuracy and Processivity, Inhibitor resistant, Thermostable
Format	Kit, Liquid
Product Category	cDNA Synthesis
Reaction Temperature	30-70 °C
Heat Inactivation	85 °C
Protocol time	Approx. 5 min.

MSDS

Material Safety Data Sheets

MSDS - EN MSDS - PT

CoA

Certificate of Analysis

Manuals

Instructions Manuals - EN

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